Fuggle S V, McWhinnie D L, Morris P J
Transplantation. 1987 Aug;44(2):214-20. doi: 10.1097/00007890-198708000-00008.
HLA-class II antigen expression is induced in the tubules of renal allografts, but it is unclear whether all three class II products--HLA-DR, DQ, and DP--are induced, and whether the induced product is of donor origin. A pretransplant (n = 14) and serial transplant biopsies (n = 45) were obtained from 14 transplant recipients in whom induced HLA-class II antigen was detected after transplantation with a monoclonal antibody reactive with HLA-DR, DP, and possibly DQ antigens. Cryostat sections were stained with locus-specific or polymorphic monoclonal antibodies in an indirect immunoperoxidase assay. In pretransplant biopsies intracellular HLA-DR antigen was expressed on proximal tubules, whereas all tubules were negative for HLA-DQ and DP products. After transplantation grafts with induced tubular HLA-class II antigen had induced HLA-DR, DQ and DP antigens expressed both within the cytoplasm and on the cell membranes. The donor or recipient origin of induced HLA-class II expression was determined using polymorphic antibodies specific for either donor or recipient antigens. This approach demonstrated that the induced class II antigen is of donor origin--and, furthermore, that the renal parenchyma remains of donor HLA-type, even one year after transplantation, and thus remains a source of antigenic stimulus to the recipient.
HLA - II类抗原在同种异体肾移植的肾小管中被诱导表达，但尚不清楚是否诱导出了所有三种II类产物——HLA - DR、DQ和DP，以及诱导产物是否来自供体。从14名移植受者身上获取了移植前（n = 14）及系列移植活检样本（n = 45），这些受者在移植后用与HLA - DR、DP以及可能的DQ抗原反应的单克隆抗体检测到了诱导的HLA - II类抗原。在间接免疫过氧化物酶测定中，用位点特异性或多态性单克隆抗体对冰冻切片进行染色。在移植前活检中，细胞内HLA - DR抗原在近端小管上表达，而所有小管的HLA - DQ和DP产物均为阴性。移植后，具有诱导性肾小管HLA - II类抗原的移植物诱导出了HLA - DR、DQ和DP抗原，它们在细胞质内和细胞膜上均有表达。使用针对供体或受体抗原的多态性抗体确定诱导的HLA - II类表达的供体或受体来源。这种方法表明，诱导的II类抗原来自供体——此外，即使在移植一年后，肾实质仍保持供体HLA型，因此仍然是对受体的抗原刺激源。
