Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Cidade Universitária, Rio de Janeiro, Brazil.
Programa Químico de Petróleo e Biocombustíveis PRH-01, Instituto de Química, Universidade Federal do Rio de Janeiro, Cidade Universitária, Rio de Janeiro, Brazil.
Int J Phytoremediation. 2021;23(4):426-435. doi: 10.1080/15226514.2020.1825324. Epub 2020 Oct 17.
L. (alfalfa) are studied as potential phytoremediation agents of priority pollutants like polycyclic aromatic hydrocarbons (PAH). However, elucidation of the biochemical mechanisms involved in phytoremediation is a topic to be explored with knowledge gaps. This study aims to identify and classify proteins expressed in the aerial parts of laboratory-cultivated alfalfa in the presence and absence of pyrene, anthracene, and phenanthrene. Soil samples were amended with 100 mg.kg of each PAH (total concentration of 300 ppm) and cultivated with alfalfa plants for 20 days. After this, aerial parts of cultivated plants from each condition were collected for qualitative proteomic analysis (ESI-Q/TOF). The results showed a significant increase (Student's -test < 0.05) of 41.7% in the concentration of proteins from plants grown in PAH-amended substrates, changes in the protein profile, with intense protein bands observed at 40-55, 34, 28, and 15 kDa when compared to the control. A total of 504 proteins were identified and classified into 12 functional categories, highlighting the identification of 11 phytoremediation-related proteins candidates in plants grown in the presence of PAH, with biological functions related to diverse metabolisms involved in the xenobiotics biodegradation (included PAH), glutathione and response to stress.
紫花苜蓿（alfalfa）被研究为多环芳烃（PAH）等优先污染物的潜在植物修复剂。然而，阐明植物修复中涉及的生化机制是一个有待探索的主题，存在知识空白。本研究旨在鉴定和分类在存在芘、蒽和菲的情况下，实验室培养的紫花苜蓿地上部分表达的蛋白质。土壤样品用 100mg.kg 的每种 PAH（总浓度为 300ppm）进行改良，并与紫花苜蓿植物一起培养 20 天。在此之后，从每种条件下培养的植物的地上部分收集进行定性蛋白质组学分析（ESI-Q/TOF）。结果表明，在添加 PAH 的基质中生长的植物的蛋白质浓度显著增加（Student's -test < 0.05），为 41.7%，蛋白质图谱发生变化，与对照相比，在 40-55、34、28 和 15kDa 处观察到强烈的蛋白质带。共鉴定出 504 种蛋白质，并分为 12 个功能类别，突出了在存在 PAH 的情况下生长的植物中鉴定出 11 种与植物修复相关的蛋白质候选物，其生物学功能与涉及异生物质生物降解（包括 PAH）、谷胱甘肽和应激反应的多种代谢有关。
