Xu Yingjie, Zheng Zhu Anzhen, Zhu Tianyuan, Zhu Bingxu, Feng Chuhan, Chen Yujia, Qin Fenju
Department of Biological Technology and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China.
Wei Sheng Yan Jiu. 2020 Sep;49(5):795-801. doi: 10.19813/j.cnki.weishengyanjiu.2020.05.018.
To study effect of nano-selenium and nano-cerium(nano cerium oxide) on the spermatogenic ability of mice irradiated by 1800 MHz microwave radiation(MR).
Forty-two ICR mice were randomly divided into groups: blank control group, solvent control group, microwave radiation model group, low, medium and high dose groups of nano-selenium+nano-cerium. In joint effects groups of nano-selenium and nano-cerium, the nano-selenium solution(60, 120 and 240 μg/kg) and the nano-cerium oxide solution(15, 30, 60 μg/kg) were administered to the stomach at 7:30 in the morning and 18:30 in the evening, respectively. The blank control group was orally administered with an equal volume of distilled water, and the solvent control group and the MR group were orally administered with an equal volume of carboxymethylcellulose sodium solution. During the second week of gastric administration, the mice were exposed to microwave radiation(1800 MHz) for 2 h every day(specific absorption ratio: 0. 2986 W/kg). After MR treatment, the daily sperm production of testis, sperm motility and sperm deformity rate in epididymis were measured, and the testicular marker enzymes [G6 PDH(6-phosphatedehydrogenase), ACP(acid phosphatase), LDH(lactate dehydrogenase)], antioxidant indexes [CAT(catalase), MDA(malondialdehyde) and T-AOC(total antioxidant capacity)] in testicular tissue were analyzed.
Compared with the solvent control group, MR led to the decrease of sperm motility and the increase of sperm deformity rate, decreased the enzymes activities of G6 PDH, ACP and CAT, increased LDH activity and MDA content, and decreased the T-AOC level in testicular tissue, and the differences were statistically significant(P<0. 05). Compared with the MR group, the joint action of nano-selenium and nano-cerium with medium dose increased the daily sperm production of testis((18. 98±1. 27) ×106/g) vs. (15. 53±1. 24) ×106/g), decreased the sperm deformity rate(11. 74%±0. 91% vs. 16. 84%±2. 05%), and the joint action of nano-selenium and nano-cerium with medium and high dose increased the sperm motility in epididymis(61. 98%±6. 33%, 54. 17±4. 38 vs. 45. 16%±5. 01%), and the differences were statistically significant(P<0. 05). Compared with the MR group, the joint action of nano-selenium and nano-cerium with low and medium dose increased the activity of ACP(11. 07±0. 98, 14. 85±1. 39 vs. 8. 72±0. 91 nmol/(min·mg prot), P<0. 05). The joint action of nano-selenium and nano-cerium with medium and high dose increased the activity of G6 PDH(24. 12±2. 06, 21. 36±3. 65 vs. 15. 11±1. 73 nmol/(min·mg prot), P<0. 05) and decreased the activity of LDH(15. 52±1. 17, 13. 51±1. 68 vs. 22. 46±2. 01 nmol/(min·mg prot), P<0. 05). The joint action of nano-selenium and nano-cerium with medium dose increased the activity of CAT(17. 92±2. 03 vs. 11. 69±0. 87 nmol/(min·mg prot), P<0. 05) and decreased the content of MDA(5. 17 ±0. 62 vs. 9. 03 ±0. 63 nmol/mg prot, P<0. 05). The joint action of nano-selenium and nano-cerium with low, medium and high dose increased the level of T-AOC(22. 06±1. 54, 29. 36±2. 39, 21. 01±2. 47 vs. 12. 88±1. 82 U/mg prot, P<0. 05).
The joint addition of nano-selenium and nano-cerium can improve the reproductive function of male mice exposed to MR, and can effectively alleviate the changes of mouse testicular marker enzyme activity and the decline of antioxidant capacity caused by MR.
研究纳米硒与纳米铈（纳米氧化铈）对1800 MHz微波辐射（MR）致小鼠生精能力的影响。
将42只ICR小鼠随机分为空白对照组、溶剂对照组、微波辐射模型组、纳米硒+纳米铈低、中、高剂量组。在纳米硒与纳米铈联合作用组中，分别于上午7:30和晚上18:30给小鼠灌胃纳米硒溶液（60、120和240 μg/kg）和纳米氧化铈溶液（15、30、60 μg/kg）。空白对照组灌胃等体积蒸馏水，溶剂对照组和MR组灌胃等体积羧甲基纤维素钠溶液。在灌胃的第二周，每天将小鼠暴露于微波辐射（1800 MHz）2小时（比吸收率：0.2986 W/kg）。MR处理后，测定睾丸每日精子生成量、附睾精子活力和精子畸形率，并分析睾丸组织中的睾丸标记酶[G6 PDH（6-磷酸脱氢酶）、ACP（酸性磷酸酶）、LDH（乳酸脱氢酶）]、抗氧化指标[CAT（过氧化氢酶）、MDA（丙二醛）和T-AOC（总抗氧化能力）]。
与溶剂对照组相比，MR导致精子活力下降、精子畸形率升高，睾丸组织中G6 PDH、ACP和CAT酶活性降低，LDH活性和MDA含量升高，T-AOC水平降低，差异有统计学意义（P<0.05）。与MR组相比，中剂量纳米硒与纳米铈联合作用增加了睾丸每日精子生成量（（18.98±1.27）×10⁶/g对（15.53±1.24）×10⁶/g），降低了精子畸形率（11.74%±0.91%对16.84%±2.05%），中、高剂量纳米硒与纳米铈联合作用增加了附睾精子活力（61.98%±6.33%，54.17±4.38对45.16%±5.01%），差异有统计学意义（P<0.05）。与MR组相比，低、中剂量纳米硒与纳米铈联合作用增加了ACP活性（11.07±0.98，14.85±1.39对8.72±0.91 nmol/（min·mg prot），P<0.05）。中、高剂量纳米硒与纳米铈联合作用增加了G6 PDH活性（24.12±2.06，21.36±3.65对15.11±1.73 nmol/（min·mg prot），P<0.05），降低了LDH活性（15.52±1.17，13.51±1.68对22.46±2.01 nmol/（min·mg prot），P<0.05）。中剂量纳米硒与纳米铈联合作用增加了CAT活性（17.92±2.03对11.69±0.87 nmol/（min·mg prot），P<0.05），降低了MDA含量（5.17±0.62对9.03±0.63 nmol/mg prot，P<0.05）。低、中、高剂量纳米硒与纳米铈联合作用增加了T-AOC水平（22.06±1.54，29.36±2.39，21.01±2.47对12.88±1.82 U/mg prot，P<0.05）。
纳米硒与纳米铈联合添加可改善受MR照射雄性小鼠的生殖功能，并能有效缓解MR引起的小鼠睾丸标记酶活性变化及抗氧化能力下降。
