非洲猪瘟病毒对猪单核细胞的细胞致病作用：特性及在微量测定中的应用。

Cytopathogenic effect of African swine fever virus for pig monocytes: characterization and use in microassay.

作者信息

Knudsen R C, Genovesi E V, Whyard T C, Wool S H

出版信息

Vet Microbiol. 1987 May;14(1):15-24. doi: 10.1016/0378-1135(87)90048-4.

Abstract

A microculture assay is described for the titration of African swine fever virus (ASFV) using swine monocytes contained in mononuclear leucocyte (MNL) microcultures. Titration endpoints were determined by observing cytopathogenic effects (CPE) of ASFV infected monocytes with an inverted microscope at 40 X magnification. CPE was a late event following the detection of ASFV antigens in monocytes by radioimmune assay, immunofluorescence and hemadsorption. It began with the detachment, enlargement and rounding of monocytes which progressively formed into grape-like clusters of 3-20 or more cells which eventually lysed. The characteristic CPE was produced in monocyte microcultures by virulent, moderately virulent, Vero cell adapted, and nonhemadsorbing ASFV strains. The sensitivity and reproducibility of the CPE microassay was similar to that of the hemadsorption microassay.

摘要

描述了一种微量培养测定法，用于使用单核白细胞（MNL）微量培养物中所含的猪单核细胞对非洲猪瘟病毒（ASFV）进行滴定。通过在40倍放大倍数下用倒置显微镜观察ASFV感染的单核细胞的细胞病变效应（CPE）来确定滴定终点。CPE是在用放射免疫测定、免疫荧光和血细胞吸附法检测到单核细胞中的ASFV抗原之后的晚期事件。它始于单核细胞的脱离、肿大和变圆，这些细胞逐渐形成3至20个或更多细胞的葡萄状簇，最终裂解。毒性、中度毒性、适应Vero细胞和非血细胞吸附性ASFV毒株在单核细胞微量培养物中产生了特征性的CPE。CPE微量测定法的灵敏度和可重复性与血细胞吸附微量测定法相似。