Wardley R C, Wilkinson P J
J Gen Virol. 1978 Jan;38(1):183-6. doi: 10.1099/0022-1317-38-1-183.
The replication of virulent African swine fever virus (ASFV) in cultures of monocytes and macrophages derived from pig bone marrow (PBM) and pig leukocyte (PL) cells was investigated by light microscopy, immunofluorescence, haemadsorption and infective virus release. Monocytes showed a high rate of infection and complete destruction within 2 to 3 days, whereas macrophages had only a very low level of infection and survived to form persistently infected cultures. These observations may explain the decrease in sensitivity of PBM and PL cells for ASFV assay after extended periods of incubation and suggest that the macrophage may be one of the cell types concerned with virus persistence in the pig.
通过光学显微镜、免疫荧光、血细胞吸附和感染性病毒释放等方法，研究了强毒非洲猪瘟病毒（ASFV）在源自猪骨髓（PBM）和猪白细胞（PL）细胞的单核细胞和巨噬细胞培养物中的复制情况。单核细胞在2至3天内显示出高感染率和完全破坏，而巨噬细胞仅有非常低的感染水平，并存活下来形成持续感染的培养物。这些观察结果可能解释了长时间孵育后PBM和PL细胞对ASFV检测敏感性的降低，并表明巨噬细胞可能是与猪体内病毒持续存在有关的细胞类型之一。
