Khiralla Ghada, Ali Hussein M
National Organization for Drug Control and Research (NODCAR), 6-7, AboHazem Street, Pyramids, PO Box 29, Giza, Egypt.
Agricultural Biochemistry Department, Faculty of Agriculture, Ain Shams University, Shubra El-Kheima, Cairo, Egypt.
J Food Sci Technol. 2020 Nov;57(11):4277-4285. doi: 10.1007/s13197-020-04467-6. Epub 2020 Apr 29.
The aim of the present work was to find the changes in the chemical composition, chemical structure and antioxidant activity of bioactive nutrients of kiwi juices upon pasteurization and in vitro gastrointestinal digestion. Results showed that fresh kiwi juice (FKJ) contains total phenols, 162 mg GAE/mL, total flavonoids 1.44 mg QE/mL, ascorbic acid 4.5 mg/mL and fiber 60%; these contents were marginally affected by pasteurization (PKJ) to be 122, 1.02, 2.6 and 47 respectively. On the other hand, gastrointestinal digestion severely lowered total phenols, total flavonoids and ascorbic acid in digested fresh kiwi juice (DFKJ) to 49 mg GAE/mL,0.16 mg GE/mL and 2.2 mg/mL, and in digested pasteurized kiwi juice (DPKJ) to 42, 0.07 and 1.2 respectively; however fiber contents increased upon digestion as a result of decreasing other nutrients. The antioxidant and reducing power activities followed the same order of decreasing total phenols, total flavonoids and ascorbic acid contents i.e. FKJ > PKJ > DFKJ > DPKJ. The major flavonoid found was apigenin in the form of various glycosides differ in the number (1-3) and position of the sugar units. Pasteurization showed minor effects on the chemical composition of fresh juice while digestion, as a result of gastrointestinal enzymes had more effect but mainly on the number and position of the sugar units rather than affecting the flavonoid moiety which preserve the main nutritive values.
本研究的目的是探究猕猴桃汁在巴氏杀菌及体外胃肠道消化过程中,生物活性营养成分的化学组成、化学结构及抗氧化活性的变化。结果表明,新鲜猕猴桃汁(FKJ)中总酚含量为162 mg GAE/mL,总黄酮含量为1.44 mg QE/mL,抗坏血酸含量为4.5 mg/mL,纤维含量为60%;经巴氏杀菌后的猕猴桃汁(PKJ)中,这些成分含量分别略有变化,变为122、1.02、2.6和47。另一方面,胃肠道消化使消化后的新鲜猕猴桃汁(DFKJ)中的总酚、总黄酮和抗坏血酸含量大幅降低,分别降至49 mg GAE/mL、0.16 mg GE/mL和2.2 mg/mL,消化后的巴氏杀菌猕猴桃汁(DPKJ)中的相应含量则分别降至42、0.07和1.2;然而,由于其他营养成分减少,消化后纤维含量增加。抗氧化和还原能力活性随总酚、总黄酮和抗坏血酸含量的降低呈现相同顺序,即FKJ>PKJ>DFKJ>DPKJ。所发现的主要黄酮类化合物为芹菜素,以各种糖苷形式存在,糖单元的数量(1 - 3个)和位置各不相同。巴氏杀菌对新鲜果汁的化学成分影响较小,而胃肠道消化酶导致的消化对其影响更大,但主要影响糖单元的数量和位置,而非影响黄酮部分,从而保留了主要营养价值。