Almdahl S M, Osterud B, Brox J H
Acta Chir Scand. 1987 Apr;153(4):287-90.
Young rats (weight 190-200 g) were subjected to splenectomy or sham laparotomy and gram-negative bacteraemia was induced by caecal perforation or by injection of viable Escherichia coli intraperitoneally (10(9) bacteria) or intravenously (2 X 10(8]. Clearance of bacteria was significantly less in the splenectomized than in the sham-laparotomized rats, irrespective of the mode of microbial inoculation. Monocyte thromboplastin activity, shown to be a sensitive indicator of gram-negative bacterial presence, was heightened in the asplenic rats. When the infectious challenge was made 15 weeks postoperatively, however, no significant difference in bacterial clearance or in monocyte thromboplastin values was found between splenectomized rats and controls. Nor was increased susceptibility to gram-negative bacteria found in asplenic rats when both operation (splenectomy vs. sham laparotomy) and bacterial challenge were performed at a late age (weight 530-580 g). The postsplenectomy clearance of gram-negative bacteria thus seemed to be age-dependent.
将年轻大鼠（体重190 - 200克）进行脾切除术或假剖腹手术，通过盲肠穿孔或腹腔注射活的大肠杆菌（10⁹个细菌）或静脉注射（2×10⁸个细菌）诱导革兰氏阴性菌血症。无论微生物接种方式如何，脾切除大鼠的细菌清除率均显著低于假剖腹手术大鼠。单核细胞凝血活酶活性是革兰氏阴性菌存在的敏感指标，在无脾大鼠中升高。然而，术后15周进行感染攻击时，脾切除大鼠与对照组之间在细菌清除率或单核细胞凝血活酶值方面未发现显著差异。当在老龄（体重530 - 580克）进行手术（脾切除术与假剖腹手术）和细菌攻击时，无脾大鼠对革兰氏阴性菌的易感性也未增加。因此，脾切除术后革兰氏阴性菌的清除似乎与年龄有关。
