Paulo Rossely, Brito Miguel, Van-Dunem Pedro, Martins António, Novak Robert J, Jacob Benjamin, Molyneux David M, Unnasch Thomas R, Stothard J Russell, Kelly-Hope Louise
Centro de Investigacao em Saude de Angola(CISA)/Health Research Centre of Angola, Caxito, Angola.
Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, UK.
Parasite Epidemiol Control. 2020 Sep 23;11:e00183. doi: 10.1016/j.parepi.2020.e00183. eCollection 2020 Nov.
The prevalence of and infections in an under-surveyed area of Bengo Province, Angola, was determined by surveying 22 communities with a combination of clinical, serological and DNA diagnostics. Additional information was collected on participants' duration of residency, access to mass drug administration, knowledge of insect vectors and use of bednets. A total of 1616 individuals (38.1% male: 61.9% female), with an average age of 43 years, were examined. For L. 6.2% ( = 100/16616) individuals were found to have eyeworm, based on the rapid assessment procedure for loiasis (RAPLOA) surveys, and 11.5% ( =178/1543) based on nested PCR analyses of venous blood. prevalences in long-term residents (>10 years) and older individuals (>60 years) were significantly higher, and older men with eyeworm were better informed about vectors. For , 4.7% ( = 74/1567) individuals were found to be positive by enzyme-linked immunosorbent assay (Ov 16 ELISA), with only three individuals reporting to have ever taken ivermectin. For no infections were found using the antigen-based immunochromatographic test (ICT) and real-time PCR analysis; however, 27 individuals presented with lymphatic filariasis (LF) related clinical conditions (lymphoedema = 11, hydrocoele = 14, both = 2). Just under half (45.5%) of the participants owned a bednet, with the majority (71.1%) sleeping under it the night before. Our approach of using combination diagnostics reveals the age-prevalence of loiasis alongside low endemicity of onchocerciasis and LF. Future research foci should be on identifying opportunities for more cost-effective ways to eliminate onchocerciasis and to develop innovative surveillance modalities for clinical LF for individual disease management and disability prevention.
通过临床、血清学和DNA诊断相结合的方法对安哥拉本戈省一个调查不足地区的罗阿丝虫病和盘尾丝虫病感染率进行了测定。还收集了参与者的居住时长、大规模药物给药情况、昆虫媒介知识以及蚊帐使用情况等额外信息。共检查了1616人（男性占38.1%：女性占61.9%），平均年龄为43岁。对于罗阿丝虫病，根据罗阿丝虫病快速评估程序（RAPLOA）调查，6.2%（n = 100/1616）的个体被发现有眼虫，而基于静脉血巢式PCR分析的结果为11.5%（n = 178/1543）。长期居民（>10年）和老年人（>60岁）中的罗阿丝虫病患病率显著更高，且有眼虫的老年男性对罗阿丝虫病媒介的了解更多。对于盘尾丝虫病，通过酶联免疫吸附测定（Ov 16 ELISA）发现4.7%（n = 74/1567）的个体呈阳性，只有3人报告曾服用过伊维菌素。对于淋巴丝虫病，使用基于抗原的免疫层析试验（ICT）和实时PCR分析未发现感染；然而，有27人出现了与淋巴丝虫病（LF）相关的临床症状（淋巴水肿 = 11例，鞘膜积液 = 14例，两者皆有 = 2例）。略低于一半（45.5%）的参与者拥有蚊帐，其中大多数（71.1%）在前一晚睡在蚊帐下。我们采用联合诊断的方法揭示了罗阿丝虫病的年龄患病率以及盘尾丝虫病和淋巴丝虫病的低流行率。未来的研究重点应是确定更具成本效益的消除盘尾丝虫病的方法，并开发针对临床淋巴丝虫病的创新监测模式，以进行个体疾病管理和预防残疾。
