Löfberg H, Grubb A, Thysell H, Nilsson E, Kjellander B, Möller C, Gruic V, Ljungquist A, Sternby N H
Acta Pathol Microbiol Immunol Scand A. 1987 Sep;95(5):297-302. doi: 10.1111/j.1699-0463.1987.tb00044_95a.x.
To determine the prevalence of renal amyloidosis of the AA-type in a defined population, formalin-fixed specimens from the kidneys of all the cases autopsied in 1983 at The General Hospital of Malmö, Sweden, were investigated using immunohistochemical techniques. Amyloid deposits of protein AA were found in 10 of 1,158 investigated cases and the calculated prevalence was 0.86 per cent. The mean age at death of the individuals with the AA-type of amyloidosis was 79 years. Six of the cases with amyloidosis had rheumatoid arthritis. The avidin-biotin-peroxidase complex technique was found to be superior to the immunofluorescence method and a high sensitivity and specificity was achieved when sequence-specific antibodies against a synthetized nonapeptide corresponding to a hydrophilic segment of the polypeptide chain of protein AA were used in the assay. Nine cases with other types of amyloid deposits in the kidneys were also detected. None of these cases showed any AA immunoreactivity but all of them demonstrated Congophilic deposits which were immunohistochemically stained by antibodies against the amyloid P-component. The prevalence of renal amyloidosis comprising all types of amyloid protein deposits was 1.64 per cent.
为确定特定人群中AA型肾淀粉样变性的患病率，我们运用免疫组化技术，对1983年在瑞典马尔默综合医院进行尸检的所有病例的肾脏福尔马林固定标本进行了研究。在1158例受调查病例中，有10例发现了AA蛋白的淀粉样沉积，计算得出的患病率为0.86%。AA型淀粉样变性患者的平均死亡年龄为79岁。其中6例淀粉样变性病例患有类风湿性关节炎。研究发现，抗生物素蛋白-生物素-过氧化物酶复合物技术优于免疫荧光法，当在检测中使用针对与AA蛋白多肽链亲水区段相对应的合成九肽的序列特异性抗体时，可实现高灵敏度和高特异性。还检测到9例肾脏有其他类型淀粉样沉积的病例。这些病例均未显示任何AA免疫反应性，但所有病例均表现出刚果红染色阳性沉积物，这些沉积物经抗淀粉样P成分抗体进行免疫组化染色。包括所有类型淀粉样蛋白沉积的肾淀粉样变性患病率为1.64%。
