tRNA 样小非编码 RNA mascRNA 促进全球蛋白质翻译。
The tRNA-like small noncoding RNA mascRNA promotes global protein translation.
机构信息
MOE Key laboratory of Bioinformatics, Cell Biology and Development Center, School of Life Sciences, Tsinghua University, Beijing, China.
State Key laboratory for Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life Sciences, Xiamen University, Fujian, China.
出版信息
EMBO Rep. 2020 Dec 3;21(12):e49684. doi: 10.15252/embr.201949684. Epub 2020 Oct 19.
Abstract
mascRNA is a small cytoplasmic RNA derived from the lncRNA MALAT1. After being processed by the tRNA processing enzymes RNase P and RNase Z, mascRNA undergoes CCA addition like tRNAs and folds into a tRNA-like cloverleaf structure. While MALAT1 functions in multiple cellular processes, the role of mascRNA was largely unknown. Here, we show that mascRNA binds directly to the multi-tRNA synthetase complex (MSC) component glutaminyl-tRNA synthetase (QARS). mascRNA promotes global protein translation and cell proliferation by positively regulating QARS protein levels. Our results uncover a role of mascRNA that is independent of MALAT1, but could be part of the molecular mechanism of MALAT1's function in cancer, and provide a paradigm for understanding tRNA-like structures in mammalian cells.
摘要
mascRNA 是一种从小核仁 RNA（lncRNA MALAT1）衍生而来的细胞质小分子 RNA。在 tRNA 加工酶 RNase P 和 RNase Z 的加工后，mascRNA 像 tRNA 一样经历 CCA 添加，并折叠成类似 tRNA 的三叶草结构。虽然 MALAT1 在多种细胞过程中发挥作用，但 mascRNA 的作用在很大程度上尚不清楚。在这里，我们表明 mascRNA 直接结合到多功能 tRNA 合成酶复合物（MSC）成分谷氨酰-tRNA 合成酶（QARS）。mascRNA 通过正向调节 QARS 蛋白水平来促进全局蛋白质翻译和细胞增殖。我们的研究结果揭示了 mascRNA 的一个独立于 MALAT1 的作用，但可能是 MALAT1 在癌症中功能的分子机制的一部分，并为理解哺乳动物细胞中类似 tRNA 的结构提供了范例。
相似文献
1
The tRNA-like small noncoding RNA mascRNA promotes global protein translation.tRNA 样小非编码 RNA mascRNA 促进全球蛋白质翻译。
EMBO Rep. 2020 Dec 3;21(12):e49684. doi: 10.15252/embr.201949684. Epub 2020 Oct 19.
2
Structural basis of MALAT1 RNA maturation and mascRNA biogenesis.MALAT1 RNA 成熟和 mascRNA 生成的结构基础。
Nat Struct Mol Biol. 2024 Nov;31(11):1655-1668. doi: 10.1038/s41594-024-01340-4. Epub 2024 Jul 2.
3
tRNA-like leader-trailer interaction promotes 3'-end maturation of MALAT1.tRNA 样的前导-尾随相互作用促进 MALAT1 的 3'-端成熟。
RNA. 2021 Oct;27(10):1140-1147. doi: 10.1261/rna.078810.121. Epub 2021 Jul 12.
4
mascRNA and its parent lncRNA MALAT1 promote proliferation and metastasis of hepatocellular carcinoma cells by activating ERK/MAPK signaling pathway.mascRNA及其亲本长链非编码RNA MALAT1通过激活ERK/MAPK信号通路促进肝癌细胞的增殖和转移。
Cell Death Discov. 2021 May 17;7(1):110. doi: 10.1038/s41420-021-00497-x.
5
The small RNA mascRNA differentially regulates TLR-induced proinflammatory and antiviral responses.小分子 RNA mascRNA 差异调节 TLR 诱导的促炎和抗病毒反应。
JCI Insight. 2021 Nov 8;6(21):e150833. doi: 10.1172/jci.insight.150833.
6
tRNA-like Transcripts from the Genomic Region Critically Influence Human Innate Immunity and Macrophage Functions.基因组区域的 tRNA 样转录本对人类先天免疫和巨噬细胞功能有重要影响。
Cells. 2022 Dec 8;11(24):3970. doi: 10.3390/cells11243970.
7
3' end processing of a long nuclear-retained noncoding RNA yields a tRNA-like cytoplasmic RNA.一种长链核滞留非编码RNA的3'端加工产生一种类似tRNA的细胞质RNA。
Cell. 2008 Nov 28;135(5):919-32. doi: 10.1016/j.cell.2008.10.012.
8
Identification and Characterization of a Class of MALAT1-like Genomic Loci.一类MALAT1样基因组位点的鉴定与表征
Cell Rep. 2017 May 23;19(8):1723-1738. doi: 10.1016/j.celrep.2017.05.006.
9
mascRNA alleviates STING-TBK1 signaling-mediated immune response through promoting ubiquitination of STING.mascRNA 通过促进 STING 的泛素化来减轻 STING-TBK1 信号转导介导的免疫反应。
Mol Immunol. 2023 Feb;154:45-53. doi: 10.1016/j.molimm.2022.12.012. Epub 2023 Jan 3.
10
Rapid RNase L-driven arrest of protein synthesis in the dsRNA response without degradation of translation machinery.在双链RNA反应中，核糖核酸酶L迅速驱动蛋白质合成停滞，而不降解翻译机制。
RNA. 2017 Nov;23(11):1660-1671. doi: 10.1261/rna.062000.117. Epub 2017 Aug 14.
引用本文的文献
1
is a -acting long noncoding RNA that enhances the host interferon response in human epithelial cells.是一种起作用的长链非编码RNA，可增强人类上皮细胞中的宿主干扰素反应。 （注：原文中“ -acting”处有缺失内容）
bioRxiv. 2025 Aug 22:2025.08.18.670784. doi: 10.1101/2025.08.18.670784.
2
Noncoding RNA and Alcohol Use Disorder: A Scoping Review of Current Research and Knowledge Gaps.非编码RNA与酒精使用障碍：当前研究及知识空白的范围综述
Alcohol Res. 2025 Jun 20;45(1):06. doi: 10.35946/arcr.v45.1.06. eCollection 2025.
3
The Secret Life of N-methyladenosine: A Review on its Regulatory Functions.N-甲基腺苷的隐秘生活：关于其调控功能的综述
J Mol Biol. 2025 Aug 15;437(16):169099. doi: 10.1016/j.jmb.2025.169099. Epub 2025 Mar 24.
4
Structural basis for tRNA mimicry by mascRNA and menRNA.mascRNA和menRNA模拟tRNA的结构基础。
Cell Discov. 2025 Jan 2;10(1):128. doi: 10.1038/s41421-024-00761-1.
5
Structural basis of MALAT1 RNA maturation and mascRNA biogenesis.MALAT1 RNA 成熟和 mascRNA 生成的结构基础。
Nat Struct Mol Biol. 2024 Nov;31(11):1655-1668. doi: 10.1038/s41594-024-01340-4. Epub 2024 Jul 2.
6
Noncoding RNA Terc-53 and hyaluronan receptor Hmmr regulate aging in mice.非编码RNA Terc-53和透明质酸受体Hmmr调节小鼠衰老。
Protein Cell. 2025 Jan 4;16(1):28-48. doi: 10.1093/procel/pwae023.
7
tRNA renovatio: Rebirth through fragmentation.tRNA 修复：通过断裂实现重生。
Mol Cell. 2023 Nov 16;83(22):3953-3971. doi: 10.1016/j.molcel.2023.09.016. Epub 2023 Oct 5.
8
The tRNA identity landscape for aminoacylation and beyond.tRNA 识别景观：氨酰化及其他功能
Nucleic Acids Res. 2023 Feb 28;51(4):1528-1570. doi: 10.1093/nar/gkad007.
9
tRNA-like Transcripts from the Genomic Region Critically Influence Human Innate Immunity and Macrophage Functions.基因组区域的 tRNA 样转录本对人类先天免疫和巨噬细胞功能有重要影响。
Cells. 2022 Dec 8;11(24):3970. doi: 10.3390/cells11243970.
10
The Long and the Short of It: and Cancer Cell Metabolism.简而言之：与癌细胞代谢
Cancers (Basel). 2022 Sep 9;14(18):4388. doi: 10.3390/cancers14184388.
本文引用的文献
1
mRNA association by aminoacyl tRNA synthetase occurs at a putative anticodon mimic and autoregulates translation in response to tRNA levels.氨酰-tRNA 合成酶通过假定的反密码子模拟物与 mRNA 结合，并根据 tRNA 水平对翻译进行自身调控。
PLoS Biol. 2019 May 17;17(5):e3000274. doi: 10.1371/journal.pbio.3000274. eCollection 2019 May.
2
HyPR-MS for Multiplexed Discovery of MALAT1, NEAT1, and NORAD lncRNA Protein Interactomes.HyPR-MS 用于 MALAT1、NEAT1 和 NORAD lncRNA 蛋白互作组的多重发现。
J Proteome Res. 2018 Sep 7;17(9):3022-3038. doi: 10.1021/acs.jproteome.8b00189. Epub 2018 Jul 31.
3
A tRNA fragment, tRF5-Glu, regulates BCAR3 expression and proliferation in ovarian cancer cells.一种tRNA片段tRF5-Glu可调节卵巢癌细胞中BCAR3的表达及增殖。
Oncotarget. 2017 Sep 8;8(56):95377-95391. doi: 10.18632/oncotarget.20709. eCollection 2017 Nov 10.
4
A transfer-RNA-derived small RNA regulates ribosome biogenesis.一种源自转运RNA的小RNA调控核糖体生物合成。
Nature. 2017 Dec 7;552(7683):57-62. doi: 10.1038/nature25005. Epub 2017 Nov 29.
5
The multidimensional mechanisms of long noncoding RNA function.长非编码 RNA 功能的多维机制。
Genome Biol. 2017 Oct 31;18(1):206. doi: 10.1186/s13059-017-1348-2.
6
Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation.定量蛋白质组学研究表明，长链非编码RNA MALAT1与DBC1相互作用以调控p53乙酰化。
Nucleic Acids Res. 2017 Sep 29;45(17):9947-9959. doi: 10.1093/nar/gkx600.
7
The long noncoding RNA Malat1: Its physiological and pathophysiological functions.长链非编码 RNA Malat1：其生理和病理生理功能。
RNA Biol. 2017 Dec 2;14(12):1705-1714. doi: 10.1080/15476286.2017.1358347. Epub 2017 Oct 6.
8
MALAT1/miR-127-5p Regulates Osteopontin (OPN)-Mediated Proliferation of Human Chondrocytes Through PI3K/Akt Pathway.MALAT1/miR-127-5p 通过 PI3K/Akt 通路调控骨桥蛋白（OPN）介导的人软骨细胞增殖。
J Cell Biochem. 2018 Jan;119(1):431-439. doi: 10.1002/jcb.26200. Epub 2017 Jun 27.
9
Identification and Characterization of a Class of MALAT1-like Genomic Loci.一类MALAT1样基因组位点的鉴定与表征
Cell Rep. 2017 May 23;19(8):1723-1738. doi: 10.1016/j.celrep.2017.05.006.
10
EPRS is a critical mTORC1-S6K1 effector that influences adiposity in mice.EPRS是一种关键的mTORC1 - S6K1效应蛋白，可影响小鼠的肥胖程度。
Nature. 2017 Feb 16;542(7641):357-361. doi: 10.1038/nature21380. Epub 2017 Feb 8.
Zotero 插件