Center for Reproductive Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.
Department of Orthopaedics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.
Birth Defects Res. 2020 Dec;112(20):1843-1849. doi: 10.1002/bdr2.1817. Epub 2020 Oct 19.
Evidences indicate that the decline of female fertility is becoming a common problem over the past few decades. Environmental exposure of Bisphenol A (BPA) has been considered as an endocrine-disrupting chemical deleteriously affecting human reproductions. To better understand the effect of BPA exposure on human ovarian granulosa cells, we treated human ovarian granulosa cell line (KGN) with increasing concentrations (0.1, 1, 10, and 100 μM) of BPA for 24 hr. About 0.1, 1, and 10 μM BPA did not significantly affect the viability of KGN while 100 μM of BPA caused a statistically significant decrease in the viability of KGN. Treatment KGN with 10 μM BPA resulted in a significant decrease in progesterone biosynthesis. The treatment also significantly increased the expression of ATP binding cassette subfamily A member 1 (ABCA1) and steroidogenic acute regulatory protein (STAR). In the current study, exposure to BPA could lead to decreased progesterone production probably through the upregulation of ABCA1 in human granulosa cells.
有证据表明，在过去几十年中，女性生育能力下降已成为一个普遍问题。双酚 A（BPA）的环境暴露已被认为是一种内分泌干扰化学物质，对人类生殖系统具有不良影响。为了更好地了解 BPA 暴露对人卵巢颗粒细胞的影响，我们用不同浓度（0.1、1、10 和 100 μM）的 BPA 处理人卵巢颗粒细胞系（KGN）24 小时。大约 0.1、1 和 10 μM 的 BPA 对 KGN 的活力没有显著影响，而 100 μM 的 BPA 导致 KGN 的活力显著下降。用 10 μM 的 BPA 处理 KGN 导致孕激素生物合成显著减少。该处理还显著增加了三磷酸腺苷结合盒 A 成员 1（ABCA1）和类固醇生成急性调节蛋白（STAR）的表达。在本研究中，BPA 的暴露可能导致孕激素产生减少，这可能是通过人颗粒细胞中 ABCA1 的上调引起的。
