BEM Service, Ramón y Cajal University Hospital - IRYCIS , Madrid, Spain.
Electromagn Biol Med. 2021 Jan 2;40(1):201-209. doi: 10.1080/15368378.2020.1830796. Epub 2020 Oct 17.
Capacitive-Resistive Electric Transfer (CRET) thermotherapies aim at tissue repair and regeneration through non-invasive application of RF currents. We have reported that the cellular response to subthermal CRET currents is non-linearly dependent on the signal frequency, and that in vitro exposure to a 448-kHz CRET signal promotes ADSC proliferation, as well as collagen and glycosaminoglycan synthesis in prechondrocytic cells. The present work investigates the response of neonatal fibroblasts to subthermal exposure (100 µA/mm) to two CRET signals: a 448-kHz, non-modulated sinusoidal wave vs. a 20-kHz amplitude-modulation of the 448-kHz carrier. To that end, cell proliferation and expression of the biomarkers Hsp47, Hsp27 and decorin were assessed by cell count, PCNA and Western blotting. The results revealed that while both signals significantly and equivalently increased early (4 h) expression of Hsp47, the modulated signal was more efficient in inducing Hsp27 and decorin overexpression and promoting cell proliferation. These data indicate that the cellular response is dependent on the RF signal modulation and suggest that the therapeutic effects of CRET could be mediated by promotion of fibroblastic proliferation and overexpression of biomarkers that are essential in skin regeneration.
电容电阻电转移 (CRET) 热疗通过无创应用射频电流来实现组织修复和再生。我们已经报道过，细胞对亚温热 CRET 电流的反应与信号频率呈非线性相关，体外暴露于 448-kHz 的 CRET 信号可促进 ADSC 的增殖，以及预软骨细胞中胶原蛋白和糖胺聚糖的合成。本研究调查了新生儿成纤维细胞对两种 CRET 信号（448-kHz 非调制正弦波和 448-kHz 载波的 20-kHz 幅度调制）的亚温热暴露（100µA/mm）的反应。为此，通过细胞计数、PCNA 和 Western blot 评估了细胞增殖和生物标志物 Hsp47、Hsp27 和核心蛋白聚糖的表达。结果表明，虽然两种信号都显著且等效地增加了早期（4 小时）Hsp47 的表达，但调制信号在诱导 Hsp27 和核心蛋白聚糖过度表达以及促进细胞增殖方面更为有效。这些数据表明细胞反应取决于射频信号的调制，并表明 CRET 的治疗效果可能通过促进成纤维细胞增殖和过度表达在皮肤再生中必不可少的生物标志物来介导。
