Erythromycin in the aquatic environment: deleterious effects on the initial development of zebrafish.

Due to the large volume of erythromycin continuously reaching waterbodies and its high persistence, this antibiotic drug has been detected in the aquatic environment at elevated concentrations. Although the problem of the presence of erythromycin in the environment is evident due to its influence in development of antimicrobial resistance, the toxicological consequences on non-target organisms remain to be determined. There are no apparent data on the impact of environmentally relevant concentrations of erythromycin on developing fish. Data on toxic effects during development are essential for evaluation of environmental risk to organisms. Therefore, the aim of this study was to investigate the effects of exposure to erythromycin on certain parameters including hatchability, survival rate, heart rate, and behavior in developing zebrafish. Zebrafish were exposed to a range of environmentally relevant concentrations of antibiotic (0.001, 0.01, 0.1, 1 μg/L) and one concentration 10-fold higher (10 μg/L). Exposure to erythromycin at 0.1 μg/L delayed hatching and decreased survival rate. Exposure to all tested concentrations increased heart rate. Further, exposure to erythromycin at 1 or 10 μg/L enhanced swimming activity. Our results indicated that erythromycin present in the aquatic environment might lead to disabling consequences in developing fish organisms and subsequently may result in ecological imbalance in the natural environment.