腕足动物Phallusia mammillata 的基因编辑与尾部神经索形成。
Gene Editing in the Ascidian Phallusia mammillata and Tail Nerve Cord Formation.
机构信息
Laboratoire de Biologie du Développement de Villefranche-sur-mer (LBDV), Sorbonne Université/CNRS, Villefranche-sur-Mer, France.
出版信息
Methods Mol Biol. 2021;2219:217-230. doi: 10.1007/978-1-0716-0974-3_13.
PMID:33074543
Abstract
Functional approaches for studying embryonic development have greatly advanced thanks to the CRISPR-Cas9 gene editing technique. Previously practiced in just a few organisms, these knockout techniques are now widely applied. Here we describe simple techniques for applying the CRISPR-Cas9 system to study the development of the nerve cord in the ascidian Phallusia mammillata.
摘要
得益于 CRISPR-Cas9 基因编辑技术，研究胚胎发育的功能方法取得了重大进展。这些敲除技术以前只在少数几种生物中进行过实践，现在已经得到了广泛应用。在这里，我们描述了将 CRISPR-Cas9 系统应用于研究腹足纲动物 Phallusia mammillata 的神经索发育的简单技术。
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