Cell and Systems Biology, University of Toronto Mississauga, Mississauga, ON, Canada.
Department of Integrative Biology, College of Biological Science, University of Guelph, Guelph, ON, Canada.
Methods Mol Biol. 2021;2219:277-288. doi: 10.1007/978-1-0716-0974-3_18.
Trichoplax adhaerens is a member of the phylum Placozoa, an enigmatic group of benthic animals with remarkably simple morphology. While initial work on these organisms has primarily focused on their morphology and the development of genomic resources, Trichoplax has received increased attention as a model for studying the evolution of nervous and sensory systems. This work is motivated by the fact that Trichoplax features distinct behaviours and responses to environmental stimuli. Therefore, much progress has been made in recent years on the molecular, cellular, and behavioral understanding of this organism. Methods outlined here provide hands-on approaches to cutting edge molecular and cellular techniques to record cellular activities in Trichoplax.
黏海绵是扁盘动物门的一个成员,这是一个具有奇特简单形态的底栖动物神秘群体。虽然对这些生物的最初研究主要集中在它们的形态和基因组资源的开发上,但随着对神经和感觉系统进化研究的增加,黏海绵也受到了更多的关注。这项工作的动机是,黏海绵具有明显的行为和对环境刺激的反应。因此,近年来在对这种生物的分子、细胞和行为理解方面取得了很大进展。这里概述的方法提供了一种动手操作的方法,用于记录黏海绵细胞活动的尖端分子和细胞技术。