Seralyzer反射光度计茶碱测定法与发射分析法之间的相关性。

Correlation between Seralyzer reflectance photometer theophylline assay and an Emit assay.

作者信息

Singh P, Lim D T, Enright T, de la Cruz R Y, Noutsis S, Lim A T, Mariano R, Chakrin A L

出版信息

Ann Allergy. 1987 Sep;59(3):176-8.

Abstract

Recently a method for theophylline assay on the Ames Seralyzer was introduced. We compared the interday variability of this method to the established interday variability of an enzyme multiplied immunoassay (Emit) technique used in the service laboratory of a hospital. Control sera and calibrator prepared to known concentrations (10, 15, and 25 micrograms/mL) were assayed singly daily for ten days with the Seralyzer machine. Assays were done singly to simulate the in-office use of the machine. Their respective coefficients of variations (CVs) were 6.96%, 5.50%, and 5.23%, which compared favorably with the established CVs for the Emit of 6.7%, 5.8%, and 6.1% respectively at comparable test concentrations. Two hundred and fifty-one sera were assayed in parallel on both machines in eight batches. Linear regression analysis of these data showed a slope of 0.995, intercept of 0.468, and a correlation coefficient of 0.945. These data suggest that the Seralyzer values compared favorably with the Emit values.

摘要

最近，一种在Ames Seralyzer上进行茶碱测定的方法被引入。我们将该方法的日间变异性与医院服务实验室使用的酶放大免疫测定（Emit）技术已确定的日间变异性进行了比较。用Seralyzer机器每天单独检测制备成已知浓度（10、15和25微克/毫升）的对照血清和校准品，共检测十天。检测单独进行以模拟该机器在办公室的使用情况。它们各自的变异系数（CV）分别为6.96%、5.50%和5.23%，在可比测试浓度下，与Emit已确定的CV分别为6.7%、5.8%和6.1%相比，情况良好。在八批次中，两台机器同时对251份血清进行了平行检测。对这些数据进行线性回归分析，结果显示斜率为0.995，截距为0.468，相关系数为0.945。这些数据表明，Seralyzer的值与Emit的值相比情况良好。