Sheen A E, Sly R M, Hite S, Giblin V R, Hebert M
Ann Allergy. 1979 Feb;42(2):77-9.
Safe, effective theophylline therapy depends on the rapid determination of serum theophylline concentrations. Two rapid methods that require less than 0.1 ml of serum are high pressure liquid chromatography (HPLC) and quantitative enzyme-multiplied immunoassay technique (EMIT). A comparison of these two methods was carried out using 117 serum samples routinely collected from asthmatic patients in Charity Hospital of Louisiana at New Orleans. After determination by the EMIT method, specimens were analyzed by HPLC in a different laboratory. Mean serum theophylline concentrations in 100 specimens containing more than trace concentrations were 14.5 mcg/ml by the EMIT method, compared with 12.1 mcg/ml by HPLC. The paired t test showed this to be a highly significant difference (p less than 0.0005). Whichever method of determination of theophylline concentration is used, frequent validation of results by comparison with those of other laboratories seems indicated.
安全、有效的茶碱治疗取决于血清茶碱浓度的快速测定。两种所需血清量少于0.1毫升的快速方法是高压液相色谱法（HPLC）和定量酶放大免疫分析技术（EMIT）。使用从新奥尔良路易斯安那慈善医院的哮喘患者常规采集的117份血清样本，对这两种方法进行了比较。通过EMIT方法测定后，标本在另一个实验室用HPLC进行分析。通过EMIT方法测定，100份含有超过痕量浓度的标本中血清茶碱平均浓度为14.5微克/毫升，而通过HPLC测定为12.1微克/毫升。配对t检验显示这是一个高度显著的差异（p小于0.0005）。无论使用哪种测定茶碱浓度的方法，似乎都需要通过与其他实验室的结果进行比较来频繁验证结果。
