Faculdade de Ciências Exatas e Tecnologia, Universidade Federal da Grande Dourados, Rodovia Dourados/Itahum, km 12. CEP 79804-970, Dourados, MS, Brazil.
Institute of Physics, Universidade Federal de Mato Grosso do Sul, CEP, 79070-900, Campo Grande, MS, Brazil.
Talanta. 2021 Jan 1;221:121611. doi: 10.1016/j.talanta.2020.121611. Epub 2020 Sep 3.
Here we present a planar polydimethylsiloxane (PDMS)-based millifluidic device in which the channel (at a serpentine-shape) and a new design of electrochemical sensor (dual-mode detection) are assembled into a single chip. The platform makes use of a fully integrated reservoir to place the reference electrode, separating it from the flowing stream. The device was properly characterized aiming to evaluate the influences of channel geometries and diameters, and the electrochemical properties were improved compared with the classical reference electrode configuration. To investigate the applicability of the proposed millifluidic device, amperometric detection was used for the analysis of tap and lake water as well as groundwater samples to determine salicylic acid - selected as a model analyte. Our experimental results have demonstrated a successful prospect as amperometric detection in PDMS-based chip and showed high tolerance to disturbance by external action provided in the use of electronic micropipette and no surface inactivation from sample-interference.
在这里，我们展示了一种基于平面聚二甲基硅氧烷（PDMS）的毫升级流控芯片，其中通道（呈蛇形）和新型电化学传感器（双模式检测）被组装到单个芯片上。该平台利用一个完全集成的储液器来放置参比电极，将其与流动的水流隔开。该器件经过适当的特性化处理，以评估通道几何形状和直径的影响，与经典的参比电极配置相比，电化学性能得到了改善。为了研究所提出的毫升级流控器件的适用性，安培检测用于分析自来水和湖水以及地下水样本中水杨酸的分析 - 水杨酸被选为模型分析物。我们的实验结果表明，在 PDMS 基片上进行安培检测具有广阔的前景，并表现出对外部作用的干扰具有很高的耐受性，这是在使用电子微量移液器时提供的，并且没有来自样品干扰的表面失活。
