Fu Ling, Wei Jinchao, Gao Yumei, Chen Rui
College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming, 650500, China.
Institute of Chemistry, The Chinese Academy of Sciences, Beijing, 100190, China.
Talanta. 2021 Jan 1;221:121635. doi: 10.1016/j.talanta.2020.121635. Epub 2020 Sep 8.
In this paper, a UPLC-ESI-MS was established to analyze macamide and macaene fractions from Lepidium meyenii (Maca). The antioxidant activity of Maca crude extract (MCE), total macamides (TMM) and total macaenes (TME) was evaluated by DPPH radical scavenging, ABTS radical scavenging and reducing power. The ability of MCE, TMM and TME against multiple cancer cell lines (leukemia HL-60, lung cancer A549, liver cancer SMMC-7721, breast cancer MCF-7, and colon cancer SW480) was used to evaluate their anticancer activity. The results demonstrate that TMM has the best free radical scavenging ability and reducing power compared to MCE, TME, and TMM, TME has the weakest antioxidant capacity compared to them. The difference in antioxidant properties between TMM and TME may be caused by benzylated alkamide in the chemical structure. TMM also has a good inhibitory effect on the five cancer cells. N-benzyl-(9Z, 12Z, 15Z)-octadecatrienamide (NBot) is the most cytotoxicity inhibitory of the macamides tested. The inhibitory effect of N-benzyl-9Z, 12Z-octadecadienamide (NBod) on colon cancer SW480 is near that of control group, while the inhibitory rates of NBot on leukemia HL-60, lung cancer A549, liver cancer SMMC-7721, and breast cancer MCF-7 are near those of the control group. Hence, NBot can be considered to have great potential as a natural drug with high efficiency and low side effects for cancer therapy.
本文建立了超高效液相色谱-电喷雾电离质谱法（UPLC-ESI-MS），用于分析玛咖（Lepidium meyenii）中的玛卡酰胺和玛卡烯组分。通过二苯基苦味酰基自由基（DPPH）清除法、2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）自由基清除法和还原能力测定，评估了玛卡粗提物（MCE）、总玛卡酰胺（TMM）和总玛卡烯（TME）的抗氧化活性。利用MCE、TMM和TME对多种癌细胞系（白血病HL-60、肺癌A549、肝癌SMMC-7721、乳腺癌MCF-7和结肠癌SW480）的作用能力，评估它们的抗癌活性。结果表明，与MCE、TME相比，TMM具有最佳的自由基清除能力和还原能力，TME的抗氧化能力最弱。TMM和TME抗氧化性能的差异可能是由化学结构中的苄基化烷酰胺引起的。TMM对这五种癌细胞也有良好的抑制作用。N-苄基-（9Z,12Z,15Z）-十八碳三烯酰胺（NBot）是所测试的玛卡酰胺中细胞毒性抑制作用最强的。N-苄基-9Z,12Z-十八碳二烯酰胺（NBod）对结肠癌SW480的抑制作用接近对照组，而NBot对白血病HL-60、肺癌A549、肝癌SMMC-7721和乳腺癌MCF-7的抑制率接近对照组。因此，NBot可被认为是一种具有高效、低副作用的癌症治疗天然药物，具有很大的潜力。
