Amano Rumi, Momoi Risa, Omata Emi, Nakahara Taiga, Kaminoyama Kaori, Kojima Mikiko, Takebayashi Yumiko, Ikematsu Shuka, Okegawa Yuki, Sakamoto Tomoaki, Kasahara Hiroyuki, Sakakibara Hitoshi, Motohashi Ken, Kimura Seisuke
RIKEN BioResource Center, Ibaraki 305-0074, Japan.
Faculty of Life Sciences, Kyoto Sangyo University, Kyoto 603-8555, Japan.
Plants (Basel). 2020 Oct 15;9(10):1372. doi: 10.3390/plants9101372.
Plants have a high regeneration capacity and some plant species can regenerate clone plants, called plantlets, from detached vegetative organs. We previously outlined the molecular mechanisms underlying plantlet regeneration from (Brassicaceae) leaf explants. However, the fundamental difference between the plant species that can and cannot regenerate plantlets from vegetative organs remains unclear. Here, we hypothesized that the viability of leaf explants is a key factor affecting the regeneration capacity of . To test this hypothesis, the viability of and leaf explants were compared, with respect to the maintenance of photosynthetic activity, senescence, and immune response. Time-course analyses of photosynthetic activity revealed that leaf explants can survive longer than those of . Endogenous abscisic acid (ABA) and jasmonic acid (JA) were found at low levels in leaf explant of . Time-course transcriptome analysis of and leaf explants suggested that senescence was suppressed at the transcriptional level in . Application of exogenous ABA reduced the efficiency of plantlet regeneration. Overall, our results propose that in nature, plant species that can regenerate in nature can survive for a long time.
植物具有很高的再生能力，一些植物物种能够从分离的营养器官再生出克隆植物，即幼苗。我们之前概述了十字花科植物叶片外植体再生幼苗的分子机制。然而，能够和不能从营养器官再生幼苗的植物物种之间的根本差异仍不清楚。在这里，我们假设叶片外植体的活力是影响植物再生能力的关键因素。为了验证这一假设，我们比较了两种植物叶片外植体在光合活性维持、衰老和免疫反应方面的活力。光合活性的时间进程分析表明，一种植物的叶片外植体比另一种植物的存活时间更长。在一种植物的叶片外植体中发现内源脱落酸（ABA）和茉莉酸（JA）水平较低。两种植物叶片外植体的时间进程转录组分析表明，一种植物在转录水平上衰老受到抑制。外源ABA的应用降低了幼苗再生的效率。总体而言，我们的结果表明，在自然界中能够再生的植物物种能够存活很长时间。
