Asia-Pacific Centre for Animal Health, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria, Australia
Asia-Pacific Centre for Animal Health, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria, Australia.
J Bacteriol. 2020 Dec 18;203(2). doi: 10.1128/JB.00154-20.
causes serious infections in ruminants, leading to huge economic losses. Lipoproteins are key components of the mycoplasma membrane and are believed to function in nutrient acquisition, adherence, enzymatic interactions with the host, and induction of the host's immune response to infection. Many genes of have not been assigned functions, in part because of their low sequence similarity with other bacteria, making it difficult to extrapolate gene functions. This study examined functions of a surface-localized leucine-rich repeat (LRR) lipoprotein encoded by of PG45. Homologs of MbfN were detected as 48-kDa peptides by Western blotting in all the strains of included in this study, with the predicted 70-kDa full-length polypeptide detected in some strains. Sequence analysis of the gene revealed the absence in some strains of a region encoding the carboxyl-terminal 147 amino acids found in strain PG45, which could account for the variation detected by immunoblotting. analysis of MbfN suggested that it may have an adhesion-related function. binding assays confirmed MbfN to be a fibronectin and heparin-binding protein. Disruption of in PG45 significantly reduced ( = 0.033) the adherence of PG45 to MDBK cells , demonstrating the role of MbfN as an adhesin. Experimental validation of the putative functions of genes in will advance our understanding of the basic biology of this economically important pathogen and is crucial in developing prevention strategies. This study demonstrated the extracellular matrix binding ability of a novel immunogenic lipoprotein of , and the role of this protein in adhesion by suggests that it could play a role in virulence.
导致反刍动物发生严重感染，造成巨大的经济损失。脂蛋白是支原体膜的重要组成部分，据信在营养物质获取、黏附、与宿主的酶相互作用以及诱导宿主对感染的免疫反应方面发挥作用。许多的基因尚未被赋予功能，部分原因是它们与其他细菌的序列相似性较低，使得很难推断基因功能。本研究检查了由 PG45 中的编码的一种表面定位亮氨酸丰富重复（LRR）脂蛋白的功能。通过 Western blot 在本研究中包含的所有 菌株中检测到 MbfN 的同源物为 48 kDa 肽，在一些菌株中检测到预测的 70 kDa 全长多肽。该基因的序列分析表明，在一些菌株中存在一个缺失的区域，该区域编码在 PG45 菌株中发现的羧基末端 147 个氨基酸，这可能是免疫印迹检测到的变异的原因。MbfN 的 分析表明它可能具有与黏附相关的功能。 结合测定证实 MbfN 是一种纤连蛋白和肝素结合蛋白。在 PG45 中破坏 显著降低了（= 0.033）PG45 对 MDBK 细胞的黏附，证明了 MbfN 作为黏附素的作用。在经济上重要的病原体中，对基因的推测功能的实验验证将推进我们对其基本生物学的理解，并且对于开发预防策略至关重要。本研究证明了一种新型免疫原性脂蛋白的细胞外基质结合能力，并且通过 表明该蛋白在黏附中的作用，它可能在毒力中发挥作用。
