Asia-Pacific Center for Animal Health, Melbourne Veterinary School, Faculty of Science, The University of Melbourne, Parkville, Victoria, Australia.
Appl Environ Microbiol. 2024 Jul 24;90(7):e0068724. doi: 10.1128/aem.00687-24. Epub 2024 Jun 12.
is an important emerging pathogen of cattle and bison, but our understanding of the genetic basis of its interactions with its host is limited. The aim of this study was to identify genes of required for interaction and survival in association with host cells. One hundred transposon-induced mutants of the type strain PG45 were assessed for their capacity to survive and proliferate in Madin-Darby bovine kidney cell cultures. The growth of 19 mutants was completely abrogated, and 47 mutants had a prolonged doubling time compared to the parent strain. All these mutants had a similar growth pattern to the parent strain PG45 in the axenic media. Thirteen genes previously classified as dispensable for the axenic growth of were found to be essential for the growth of in association with host cells. In most of the mutants with a growth-deficient phenotype, the transposon was inserted into a gene involved in transportation or metabolism. This included genes coding for ABC transporters, proteins related to carbohydrate, nucleotide and protein metabolism, and membrane proteins essential for attachment. It is likely that these genes are essential not only but also for the survival of in infected animals.
causes chronic bronchopneumonia, mastitis, arthritis, keratoconjunctivitis, and reproductive tract disease in cattle around the globe and is an emerging pathogen in bison. Control of mycoplasma infections is difficult in the absence of appropriate antimicrobial treatment or effective vaccines. A comprehensive understanding of host-pathogen interactions and virulence factors is important to implement more effective control methods against . Recent studies of other mycoplasmas with cell culture models have identified essential virulence genes of mycoplasmas. Our study has identified genes of required for survival in association with host cells, which will pave the way to a better understanding of host-pathogen interactions and the role of specific genes in the pathogenesis of disease caused by .
是牛和野牛的一种重要的新兴病原体，但我们对其与宿主相互作用的遗传基础的了解有限。本研究的目的是鉴定与宿主细胞相互作用和存活相关的 所需的基因。评估了 100 个转座子诱导的 PG45 标准株突变体在马迪-达比牛肾细胞培养物中存活和增殖的能力。19 个突变体的生长完全被阻断，47 个突变体的倍增时间比亲本菌株延长。与亲本菌株 PG45 相比，所有这些突变体在无细胞培养基中的生长模式相似。以前被归类为 非必需的 13 个基因对于与宿主细胞共同生长的 生长是必需的。在大多数生长缺陷表型的突变体中，转座子插入到参与运输或代谢的基因中。这包括 ABC 转运蛋白、与碳水化合物、核苷酸和蛋白质代谢相关的蛋白质以及对附着至关重要的膜蛋白的编码基因。这些基因不仅对 而且对感染动物中 的存活可能是必需的。
在全球范围内引起牛的慢性支气管肺炎、乳腺炎、关节炎、角膜炎和生殖道疾病，并且是野牛中的一种新兴病原体。在缺乏适当的抗菌治疗或有效的疫苗的情况下，很难控制支原体感染。全面了解宿主-病原体相互作用和毒力因子对于实施更有效的控制方法来对抗 非常重要。最近使用 细胞培养模型对其他支原体的研究已经确定了支原体的必需毒力基因。我们的研究鉴定了与宿主细胞共同生存所需的 基因，这将为更好地了解宿主-病原体相互作用以及特定基因在由 引起的疾病发病机制中的作用铺平道路。
