Safarík I
Biomed Biochim Acta. 1987;46(4):293-6.
Trypsin and extracellular proteinases produced by Bacillus sp. were purified by column chromatography on coffee grain particles. The ballast proteins were eluted with water, while the adsorbed proteinases were eluted with 1 M sodium chloride solution. The capacity is approximately 2 mg of trypsin per ml of the packed sorbent.
通过在咖啡颗粒上进行柱色谱法，对芽孢杆菌属产生的胰蛋白酶和细胞外蛋白酶进行了纯化。用洗脱填充吸附剂中大约每毫升2毫克的胰蛋白酶。用蒸馏水将杂质蛋白洗脱，而用1M氯化钠溶液洗脱吸附的蛋白酶。
