利用染料配体色谱法快速纯化两种嗜热蛋白酶。
Rapid purification of two thermophilic proteinases using dye-ligand chromatography.
作者信息
Cowan D A, Daniel R M
机构信息
Thermophile Research Unit, University of Waikato, Hamilton, New Zealand.
出版信息
J Biochem Biophys Methods. 1996 Jan 11;31(1-2):31-7. doi: 10.1016/0165-022x(95)00028-p.
PMID:8926336
Abstract
Dye-ligand chromatography has been used successfully for the purification of extracellular thermostable proteinases from thermophilic Bacillus and Thermus cultures. Single step purification factors of up to 115-fold (for Thermus protease) and 2195-fold (for Bacillus protease) were obtained. Elution studies suggested that the mode of binding involved the enzyme active sites. The method was readily scaleable to 600 1 volume.
摘要
染料配体色谱法已成功用于从嗜热芽孢杆菌和嗜热栖热菌培养物中纯化细胞外热稳定蛋白酶。获得了高达115倍（嗜热栖热菌蛋白酶）和2195倍（芽孢杆菌蛋白酶）的单步纯化因子。洗脱研究表明，结合模式涉及酶活性位点。该方法易于扩大到600升体积。
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