从生物安全 3 级实验室中去除高致病性病原微生物样本的已验证方法。
Validated Methods for Removing Select Agent Samples from Biosafety Level 3 Laboratories.
出版信息
Emerg Infect Dis. 2020 Nov;26(11):2586-2590. doi: 10.3201/eid2611.191630.
Abstract
The Federal Select Agent Program dictates that all research entities in the United States must rigorously assess laboratory protocols to sterilize samples being removed from containment areas. We validated procedures using sterile filtration and methanol to remove the following select agents: Francisella tularensis, Burkholderia pseudomallei, B. mallei, Yersinia pestis, and Bacillus anthracis. We validated methanol treatment for B. pseudomallei. These validations reaffirm safety protocols that enable researchers to keep samples sufficiently intact when samples are transferred between laboratories.
摘要
联邦选择代理人计划规定，美国所有的研究实体都必须严格评估实验室方案，以对从隔离区取出的样本进行消毒。我们使用无菌过滤和甲醇验证了以下选择剂的程序：土拉弗朗西斯菌、鼻疽伯克霍尔德菌、马鼻疽伯克霍尔德菌、鼠疫耶尔森菌和炭疽芽孢杆菌。我们验证了甲醇处理鼻疽伯克霍尔德菌的方法。这些验证再次确认了安全协议，使研究人员在实验室之间转移样本时能够保持样本的完整性。
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