Huaxi MR Research Center, Department of Nuclear Medicine, Frontiers Science Center for Disease-related Molecular Network, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, 610041, China.
Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, Brisbane, QLD, 4072, Australia.
Angew Chem Int Ed Engl. 2021 Feb 1;60(5):2393-2397. doi: 10.1002/anie.202011544. Epub 2020 Dec 1.
Highly fluorogenic tetrazine bioorthogonal probes emitting at near-infrared wavelengths are in strong demand for biomedical imaging applications. Herein, we have developed a strategy for forming a palette of novel Huaxi-Fluor probes in situ, whose fluorescence increases hundreds of times upon forming the bioorthogonal reaction product, pyridazine. The resulting probes show large Stokes shifts and high quantum yields. Manipulating the conjugate length and pull-push strength in the fluorophore skeleton allows the emission wavelength to be fine-tuned from 556 to 728 nm. The highly photo-stable and biocompatible probes are suitable for visualizing organelles in live cells without a washing step and for imaging of tumors in live small animals to depths of 500 μm by two-photon excitation.
高度荧光的四嗪生物正交探针在近红外波长处发射，强烈需求用于生物医学成像应用。在此，我们开发了一种原位形成新型华西荧光探针的策略，其荧光在形成生物正交反应产物哒嗪后增加数百倍。所得探针具有大的斯托克斯位移和高的量子产率。通过控制荧光团骨架中的共轭长度和推拉强度，可以将发射波长从 556 纳米精细调谐至 728 纳米。这些高稳定性和生物相容性的探针适用于在活细胞中可视化细胞器，而无需洗涤步骤，并且可以通过双光子激发在活体小动物中对 500 μm 的深度进行肿瘤成像。
