Jones R J, Sharkis S J, Celano P, Colvin O M, Rowley S D, Sensenbrenner L L
Johns Hopkins Oncology Center, Johns Hopkins Medical Institutions, Baltimore, MD 21205.
Blood. 1987 Oct;70(4):1186-92.
Hematopoietic reconstitution following syngeneic bone marrow transplantation with graded doses of untreated and drug-treated bone marrow was studied in B6D2F1 mice. Granulocyte-macrophage colony-forming units (CFU-GM) and spleen colony-forming units (CFU-S) showed similar in vitro drug sensitivities. Both the speed of hematologic recovery and survival of mice transplanted with untreated or drug-treated bone marrow were directly related to the number of CFU-GM or CFU-S transplanted. Similar hematologic recovery was seen for untreated marrow transplants and treated transplants that had similar CFU-GM or CFU-S content. There is a minimum number of transplanted CFU-GM or CFU-S that allows survival of lethally irradiated mice. This number is present in a marrow transplant containing the equivalent of 5 X 10(3) untreated cells or producing one to two spleen colonies. There also exists a maximum value for the number of hematopoietic progenitors in a marrow graft, above which the rate of hematologic recovery following transplantation is rapid and no detectable increase in the rate is seen with increasing CFU-GM or CFU-S content. The presence of this maximum value for transplanted progenitors and variations in culture techniques are probably the reasons previous studies have not always shown a correlation between CFU-GM content and hematologic recovery after bone marrow transplantation.
在B6D2F1小鼠中研究了用不同剂量未经处理和经药物处理的同基因骨髓进行同基因骨髓移植后的造血重建。粒细胞-巨噬细胞集落形成单位（CFU-GM）和脾集落形成单位（CFU-S）显示出相似的体外药物敏感性。接受未经处理或经药物处理骨髓移植的小鼠的血液学恢复速度和生存率均与移植的CFU-GM或CFU-S数量直接相关。对于CFU-GM或CFU-S含量相似的未经处理的骨髓移植和经处理的移植，观察到相似的血液学恢复情况。存在一个最低数量的移植CFU-GM或CFU-S，可使接受致死性照射的小鼠存活。这个数量存在于含有相当于5×10³个未经处理细胞或能产生一到两个脾集落的骨髓移植中。骨髓移植物中造血祖细胞的数量也存在一个最大值，超过此值，移植后的血液学恢复速度很快，并且随着CFU-GM或CFU-S含量的增加，恢复速度没有可检测到的增加。移植祖细胞存在这个最大值以及培养技术的差异可能是先前研究并不总是显示CFU-GM含量与骨髓移植后血液学恢复之间存在相关性的原因。
