Clínica Oftalmológica García de Oteyza, Barcelona, Spain; and.
Cornea and Refractive Surgery Department, Asociación Para Evitar la Ceguera en México, Hospital "Luis Sánchez Bulnes," México City, México.
Cornea. 2021 May 1;40(5):664-668. doi: 10.1097/ICO.0000000000002561.
To report the in vivo confocal microscopy (IVCM) findings of a case who underwent femtosecond laser-assisted Bowman layer transplantation (BLT).
This is a case report.
In a 25-year-old man with previous diagnosis of keratoconus in whom a BLT was performed using the femtosecond laser-assisted technique, IVCM (Confoscan 4; Fortune Technologies, Vigonza, Italy) was performed 3 months after surgery, obtaining a sequence of 4 digitalized images of the total thickness of the cornea, from the epithelium to the endothelium, and then backward. The images were analyzed with the Navis v. 3.5.0. system (NIDEK, Multi-Instrument Diagnostic System, Japan). In the anterior stroma, before arriving to the graft interphase, activated keratocytes could be observed with higher reflectivity. In this first image of the anterior interphase, a homogenous hyporreflective image could be observed. In the interior of the graft, no keratocytes were found and some hyperreflective particles were present. In addition to the anterior, the posterior interphase presented bright particles.
This is the first report that documents a BLT using IVCM.
报告一例接受飞秒激光辅助板层间角膜移植术（BLT）的患者的活体共聚焦显微镜（IVCM）检查结果。
这是一个病例报告。
一名 25 岁男性，先前被诊断为圆锥角膜，使用飞秒激光辅助技术进行了 BLT，术后 3 个月进行 IVCM（Confoscan 4；Fortune Technologies，Vigonza，意大利），共采集了 4 张角膜总厚度的数字化图像，从上皮到内皮，然后再向后。使用 Navis v.3.5.0 系统（NIDEK，多仪器诊断系统，日本）对图像进行分析。在前基质中，在到达移植物界面之前，可以观察到活性更高的角膜细胞，反射率更高。在前界面的第一幅图像中，可以观察到均匀的低反射图像。在移植物内部，未发现角膜细胞，存在一些高反射性颗粒。除了前部界面，后部界面也呈现出明亮的颗粒。
这是首例使用 IVCM 记录 BLT 的报告。
