Department of Chemistry and Biochemistry, The University of Texas at Dallas, Richardson, TX 75080, USA.
Bioinformatics. 2021 Jul 27;37(13):1926-1927. doi: 10.1093/bioinformatics/btaa892.
Hydrogen-Deuterium eXchange coupled to mass spectrometry is a powerful tool for the analysis of protein dynamics and interactions. Bottom-up experiments looking at deuterium uptake differences between various conditions are the most common. These produce multi-dimensional data that can be challenging to depict in a single visual format. Each user must also set significance thresholds to define meaningful differences and make these apparent in data presentation. To assist in this process, we have created HD-eXplosion, an open-source, web-based application for the generation of chiclet and volcano plots with statistical filters. HD-eXplosion fills a void in available software packages and produces customizable plots that are publication quality.
The HD-eXplosion application is available at http://hd-explosion.utdallas.edu. The source code can be found at https://github.com/HD-Explosion.
氢氘交换结合质谱分析是研究蛋白质动态和相互作用的有力工具。最常见的是针对不同条件下氘摄入差异的自上而下的实验。这些实验产生多维数据，在单一可视化格式中难以描绘。每个用户还必须设置显著性阈值来定义有意义的差异，并在数据表示中使其明显。为了协助这一过程，我们创建了 HD-eXplosion，这是一个开源的、基于网络的应用程序，用于生成带有统计筛选器的香豆素和火山图。HD-eXplosion 填补了现有软件包中的空白，并生成了可定制的、具有出版质量的图。
HD-eXplosion 应用程序可在 http://hd-explosion.utdallas.edu 上获得。源代码可在 https://github.com/HD-Explosion 上找到。
