Universidade do Estado de Minas Gerais, Avenida Juca Stockler, 1130, CEP 37900-106, Nossa Sra. das Graças, Passos, Minas Gerais, Brazil.
Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Avenida Presidente Antônio Carlos, 6627, Departamento de Parasitologia, L4 237, Laboratório de Parasitologia Molecular, Pampulha, CEP 31270-901, Belo Horizonte, Minas Gerais, Brazil.
Infect Genet Evol. 2020 Dec;86:104594. doi: 10.1016/j.meegid.2020.104594. Epub 2020 Oct 17.
Necator americanus is a worm that parasites the small intestine of humans and is highly prevalent in regions with poor sanitary conditions. The main strategy to control this helminth is by mass benzimidazole administration, however, periodic use of these drugs can select strains of parasites resistant to treatment. Single nucleotide polymorphisms (SNPs) in the beta-tubulin isotype 1 gene located at codons 167, 198 and 200 have been associated with benzimidazole resistance in some nematodes. Previously, our group detected the presence of some of these SNPs in populations of soil-transmitted helminths collected in different locations in Brazil. Here, we evaluated the SNP at codon 167, which has recently been shown to be associated with failure of benzimidazoles to treat N. americanus. Our ARMS-PCR analyses were performed using 524 single N. americanus eggs from 48 patients' feces collected in six Brazilian states; however, we did not detect any mutated samples at codon 167. This study builds on previous work, helping us monitor the presence of resistance-related genotypes in Brazilian helminth populations. The data presented here can assist in the implementation of future control strategies.
美洲板口线虫是一种寄生在人类小肠内的蠕虫，在卫生条件差的地区非常普遍。控制这种寄生虫的主要策略是大规模使用苯并咪唑类药物，但这些药物的定期使用会选择对治疗有抗性的寄生虫株。位于 167、198 和 200 密码子的β-微管蛋白同型 1 基因中的单核苷酸多态性 (SNP) 与一些线虫对苯并咪唑类药物的抗性有关。以前，我们的研究小组在巴西不同地区采集的土壤传播性蠕虫种群中检测到了其中一些 SNP 的存在。在这里，我们评估了最近与苯并咪唑类药物治疗美洲板口线虫失败相关的 167 密码子 SNP。我们使用来自巴西六个州的 48 名患者粪便中采集的 524 个单个美洲板口线虫卵进行了 ARMS-PCR 分析，但未在 167 密码子检测到任何突变样本。这项研究建立在前一项工作的基础上，有助于我们监测巴西寄生虫种群中与耐药性相关的基因型的存在。这里提供的数据可以为未来的控制策略提供帮助。
