Institute of Parasitology, McGill University, Ste. Anne-de-Bellevue, Canada.
Am J Trop Med Hyg. 2013 Jun;88(6):1052-61. doi: 10.4269/ajtmh.12-0484. Epub 2013 Mar 4.
In endemic countries with soil-transmitted helminths mass drug administration with albendazole or mebendazole are being implemented as a control strategy. However, it is well known in veterinary helminths that the use of the same benzimidazole drugs can place selection on the β-tubulin gene, leading to resistance. Given the concern that resistance could arise in human soil-transmitted helminths, there is an urgent need to develop accurate diagnostic tools for monitoring resistance. In this study, we developed molecular assays to detect putative resistance genetic changes in Ascaris lumbricoides, Trichuris trichiura, and hookworms, and we optimized an egg hatch assay for the canine hookworm Ancylostoma caninum and applied it to Necator americanus. Both assays were tested on field samples. The molecular assays demonstrated their reproducibility and capacity to detect the presence of worms carrying putative resistance-associated genetic changes. However, further investigations are needed to validate our molecular and biological tests on additional field isolates.
在流行土源性蠕虫的国家，阿苯达唑或甲苯达唑的群体药物治疗被用作一种控制策略。然而，兽医蠕虫学中众所周知，使用相同的苯并咪唑类药物会对β-微管蛋白基因产生选择作用，导致耐药性。鉴于人们担心人类土源性蠕虫可能会产生耐药性，因此迫切需要开发准确的诊断工具来监测耐药性。在这项研究中，我们开发了分子检测方法来检测蛔虫、鞭虫和钩虫中可能存在的耐药性遗传变化，并对犬钩虫Ancylostoma caninum 进行了卵孵化检测优化，并将其应用于美洲板口线虫 Necator americanus 。这两种检测方法都在现场样本中进行了测试。分子检测方法证明了它们的重现性和检测携带耐药相关遗传变化的蠕虫存在的能力。然而，还需要进一步的研究来验证我们的分子和生物学检测方法在其他现场分离株上的有效性。
