Innovation Center for Holistic Health, Nutraceuticals and Cosmeceuticals, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.
Department of Microbiology, Division of Biological Science, Faculty of Science, Prince of Songkla University, Hat Yai 90112, Thailand.
Molecules. 2020 Oct 18;25(20):4784. doi: 10.3390/molecules25204784.
Dextranase catalyzes the degradation of the substrate dextran, which is a component of plaque biofilm. This enzyme is involved in antiplaque accumulation, which can prevent dental caries. The activity of crude dextranase from TISTR 3511 was assessed, and the maximum value (7.61 unit/g) was obtained at 37 °C and pH 6. The Plackett-Burman design was used to obtain significant factors for enhancing fungal dextranase production, and three influencing factors were found: Dextran, yeast extract concentration and inoculum age. Subsequently, the significant factors were optimized with the Box-Behnken design, and the most suitable condition for dextranase activity at 30.24 unit/g was achieved with 80 g/L dextran, 30 g/L yeast extract and five day- old inoculum. The use of 0.85% alginate beads for encapsulation exhibited maximum dextranase activity at 25.18 unit/g beads, and this activity was stable in toothpaste for three months of testing. This study explored the potential production of fungal dextranase under optimal conditions and its encapsulation using alginate for the possibility of applying encapsulated dextranase as an additive in toothpaste products for preventing dental caries.
葡聚糖酶催化底物葡聚糖的降解，葡聚糖是菌斑生物膜的组成部分。这种酶参与抗菌斑积聚，从而预防龋齿。评估了 TISTR 3511 的粗葡聚糖酶的活性，在 37°C 和 pH6 时获得最大值（7.61 单位/g）。使用 Plackett-Burman 设计获得了增强真菌葡聚糖酶生产的显著因素，发现了三个影响因素：葡聚糖、酵母提取物浓度和接种龄。随后，使用 Box-Behnken 设计对显著因素进行了优化，在 30.24 单位/g 葡聚糖酶活性下，最适条件为 80 g/L 葡聚糖、30 g/L 酵母提取物和 5 天龄接种物。使用 0.85%海藻酸钠珠进行包封，在 25.18 单位/g 珠中显示出最大的葡聚糖酶活性，并且在三个月的牙膏测试中这种活性稳定。本研究探索了在最佳条件下真菌葡聚糖酶的潜在生产及其用海藻酸钠包封的可能性，将包封的葡聚糖酶作为预防龋齿的牙膏产品添加剂的可能性。
