Masters B S, Stohl L L, Clayton D A
Department of Pathology, Stanford University School of Medicing, California 94305-5324.
Cell. 1987 Oct 9;51(1):89-99. doi: 10.1016/0092-8674(87)90013-4.
Analysis of the nucleotide sequence of the genetic locus for yeast mitochondrial RNA polymerase (RPO41) reveals a continuous open reading frame with the coding potential for a polypeptide of 1351 amino acids, a size consistent with the electrophoretic mobility of this enzymatic activity. The transcription product from this gene spans the singular reading frame. In vivo transcript abundance reflects codon usage and growth under stringent conditions for mitochondrial biogenesis and function results in a several fold higher level of gene expression than growth under glucose repression. A comparison of the yeast mitochondrial RNA polymerase amino acid sequence to those of E. coli RNA polymerase subunits failed to demonstrate any regions of homology. Interestingly, the mitochondrial enzyme is highly homologous to the DNA-directed RNA polymerases of bacteriophages T3 and T7, especially in regions most highly conserved between the T3 and T7 enzymes themselves.
对酵母线粒体RNA聚合酶（RPO41）基因位点的核苷酸序列分析显示，有一个连续的开放阅读框，其编码潜力为一个由1351个氨基酸组成的多肽，该大小与这种酶活性的电泳迁移率一致。该基因的转录产物跨越单一阅读框。体内转录本丰度反映密码子使用情况，并且在严格的线粒体生物发生和功能条件下生长时，基因表达水平比在葡萄糖抑制下生长时高几倍。将酵母线粒体RNA聚合酶的氨基酸序列与大肠杆菌RNA聚合酶亚基的序列进行比较，未发现任何同源区域。有趣的是，线粒体酶与噬菌体T3和T7的DNA指导的RNA聚合酶高度同源，尤其是在T3和T7酶本身之间最保守的区域。
