引进的美国蔓越莓（艾顿）果实中酚类化合物的定性、定量组成及含量的变异性
Variability in the Qualitative and Quantitative Composition and Content of Phenolic Compounds in the Fruit of Introduced American Cranberry ( Aiton).
作者信息
Gudžinskaitė Ieva, Stackevičienė Elicija, Liaudanskas Mindaugas, Zymonė Kristina, Žvikas Vaidotas, Viškelis Jonas, Urbštaitė Rima, Janulis Valdimaras
机构信息
Department of Pharmacognosy, Faculty of Pharmacy, Lithuanian University of Health Sciences, LT-50166 Kaunas, Lithuania.
Nature Research Center, Institute of Botany, LT-08406 Vilnius, Lithuania.
出版信息
Plants (Basel). 2020 Oct 16;9(10):1379. doi: 10.3390/plants9101379.
The aim of this study was to determine the composition and content of phenolic compounds in ethanol extracts of eight different cultivars of American cranberry ( Aiton) fruit using spectrophotometric and UPLC-ESI-MS/MS analysis and to evaluate the antioxidant activity in vitro of these extracts. The highest total amount of phenolic compounds evaluated via Folin-Ciocalteu spectrophotometry was detected in American cranberry fruit samples of the 'Bain' clone, and the highest total amount of flavonoids was found in samples of the 'Drever' and 'Baiwfay' cultivars. The highest total amount of the individual phenolic compounds (519.53 ± 25.12 mg/g DW) identified and quantitatively evaluated via chromatography was detected in samples of the 'Searles' cranberry cultivar. In the studied cranberry samples, the predominant phenolic compounds were hyperoside, quercetin, and procyanidin A2, while the amounts of other compounds were significantly lower. HCA and PCA revealed that 'Woolman', 'Holliston', 'Pilgrim, and 'Searles' fruit samples had different quantitative content of phenolic compounds from other cranberry cultivars. Meanwhile, fruit of 'Baiwfay', 'Drever', 'Bain', and 'Bergman' were similar in their phytochemical profile.
本研究的目的是使用分光光度法和超高效液相色谱-电喷雾串联质谱分析法（UPLC-ESI-MS/MS）测定八个不同品种美国蔓越莓（Aiton）果实乙醇提取物中酚类化合物的组成和含量，并评估这些提取物的体外抗氧化活性。通过福林-西奥尔特（Folin-Ciocalteu）分光光度法评估，在‘Bain’克隆的美国蔓越莓果实样品中检测到酚类化合物的总量最高，在‘Drever’和‘Baiwfay’品种的样品中发现黄酮类化合物的总量最高。通过色谱法鉴定并定量评估的单个酚类化合物总量最高（519.53±25.12毫克/克干重），在‘Searles’蔓越莓品种的样品中检测到。在所研究的蔓越莓样品中，主要的酚类化合物是金丝桃苷、槲皮素和原花青素A2,而其他化合物的含量则显著较低。主成分分析（HCA）和主成分分析（PCA）显示，‘Woolman’、‘Holliston’、‘Pilgrim’和‘Searles’果实样品中酚类化合物的定量含量与其他蔓越莓品种不同。同时，‘Baiwfay’、‘Drever’、‘Bain’和‘Bergman’的果实具有相似的植物化学特征。
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