Liu Yang, Xiong Miao, Zhou Fangfang, Shi Nana, Jia Yunbin
Shanghai Sixth People's Hospital East Affiliated to Shanghai University of Medicine & Health Sciences, Shanghai, China.
J Int Med Res. 2020 Oct;48(10):300060520934288. doi: 10.1177/0300060520934288.
Baicalin is a compound extracted from the dried root of Georgi. Studies have shown that baicalin has a protective effect on vascular endothelial cells, but whether baicalin could alleviate ascular endothelial cell damage in pregnancy-induced hypertensive patients remains unknown.
We established a hypertensive pregnant rat model to study vascular endothelial injury during pregnancy-induced hypertension. Plasma epoprostenol (PGI-2), thromboxane A2 (Txa-2), β-human chorionic gonadotropin (β-HCG), and estrogen levels in rats were detected using ELISA. Vascular endothelial growth factor (VEGF), endothelial nitric oxide synthase (eNOS), and C-reactive protein (CRP) expression were detected using western blotting and quantitative PCR (q-PCR).
Results showed that baicalin alleviated symptoms of pregnancy-induced hypertension. CRP, Txa-2, and β-HCG expression were significantly upregulated, while VEGF, eNOS, PGI-2, and estrogen expression was decreased in plasma and placental tissues of hypertensive rats. However, the levels of these injury indicators were significantly decreased after baicalin therapy, while the expression of protective indicators was significantly increased.
Baicalin reversed vascular endothelial cell injury in pregnant hypertensive rats by promoting VEGF, eNOS, PGI-2, and estrogen expression.
黄芩苷是从黄芩干燥根中提取的一种化合物。研究表明，黄芩苷对血管内皮细胞具有保护作用，但黄芩苷是否能减轻妊娠高血压患者的血管内皮细胞损伤尚不清楚。
我们建立了妊娠高血压大鼠模型，以研究妊娠高血压期间的血管内皮损伤。采用酶联免疫吸附测定法（ELISA）检测大鼠血浆中依前列醇（PGI-2）、血栓素A2（Txa-2）、β-人绒毛膜促性腺激素（β-HCG）和雌激素水平。采用蛋白质免疫印迹法和定量聚合酶链反应（q-PCR）检测血管内皮生长因子（VEGF）、内皮型一氧化氮合酶（eNOS）和C反应蛋白（CRP）的表达。
结果显示，黄芩苷减轻了妊娠高血压症状。高血压大鼠血浆和胎盘组织中CRP、Txa-2和β-HCG表达显著上调，而VEGF、eNOS、PGI-2和雌激素表达降低。然而，黄芩苷治疗后这些损伤指标水平显著降低，而保护指标的表达显著增加。
黄芩苷通过促进VEGF、eNOS、PGI-2和雌激素表达，逆转了妊娠高血压大鼠的血管内皮细胞损伤。
