Department of Immunology, Adolfo Lutz Institute, São Paulo, SP, Brazil; Post-Graduate Program Interunities in Biotechnology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil.
Embrapa Southern Region Animal Husbandry, Bagé, RS, Brazil.
Vaccine. 2020 Nov 10;38(48):7674-7682. doi: 10.1016/j.vaccine.2020.09.081. Epub 2020 Oct 17.
Outer membrane vesicles (OMVs) of Neisseria meningitidis contain important antigens to trigger an immune response against meningococci and have been studied as vaccines compounds. The immune response to a vaccine may be affected by its constitution and route of administration. Therefore, Swiss mice were immunized by different routes with OMVs of N. meningitidis B with dimethyl dioctadecyl ammonium bromide in bilayer fragments (DDA-BF) or aluminum hydroxide (AH) as adjuvants. The adjuvants and different routes were compared regarding the immune responses by ELISA, western blot, delayed type hypersensitivity (DTH) and histopathologic analysis. The antigenic preparation generated humoral and cellular immune responses. In quantitative analyzes, in general, AH was superior to DDA-BF. However, analysis such as IgG avidity index, bactericidal activity and immunoblot, revealed no important differences regarding the adjuvant or route of immunization. Regarding the parameters tested, it was not possible to define a superiority between the adjuvants and routes of immunization proposed by this study.
脑膜炎奈瑟菌外膜囊泡（OMVs）含有重要的抗原，可引发针对脑膜炎奈瑟菌的免疫反应，已被研究作为疫苗化合物。疫苗的免疫反应可能受到其组成和给药途径的影响。因此，瑞士小鼠通过不同途径用二甲基双十八烷基溴化铵双层片段（DDA-BF）或氢氧化铝（AH）佐剂免疫脑膜炎奈瑟菌 B 的 OMVs。通过 ELISA、western blot、迟发型超敏反应（DTH）和组织病理学分析比较了佐剂和不同途径的免疫反应。抗原制剂产生体液和细胞免疫反应。在定量分析中，一般来说，氢氧化铝优于二甲基双十八烷基溴化铵。然而，对 IgG 亲和力指数、杀菌活性和免疫印迹的分析表明，佐剂或免疫途径没有重要差异。就所测试的参数而言，本研究提出的佐剂和免疫途径之间不可能确定优势。
