Dutta Swarnalee, Yu Sang-Mi, Lee Yong Hoon
Division of Biotechnology, Jeonbuk National University, Iksan 55496, Korea.
Freshwater Bioresources Utilization Division, Nakdonggang National Institute of Biological Resources, Sangju 54596, Korea.
Plant Pathol J. 2020 Oct 1;36(5):491-496. doi: 10.5423/PPJ.FT.08.2020.0149.
An understanding of the contribution of secondary metabolites (SMs) to the antagonistic and biocontrol activities of bacterial biocontrol agents serves to improve biocontrol potential of the strain. In this study, to evaluate the contribution of each SM produced by NBC275 (Pf275) to its antifungal and biocontrol activity, we combined analysis of the genome with our previous study of transposon (Tn) mutants. Thirteen Tn mutants, which belonged to 6 biosynthetic gene clusters (BGCs) of a total 14 BGCs predicted by the antiSMASH tool were identified by the reduction of antifungal activity. The biocontrol performance of Pf275 was significantly dependent on 2,4-diacetylphloroglucinol and pyoverdine. The clusters that encode for arylpolyene and an unidentified small linear lipopeptide influenced antifungal and biocontrol activities. To our knowledge, our study identified the contribution of SMs, such as a small linear lipopeptide and arylpolyene, to biocontrol efficacy for the first time.
了解次生代谢产物（SMs）对细菌生防菌拮抗和生防活性的贡献有助于提高菌株的生防潜力。在本研究中，为了评估NBC275（Pf275）产生的每种SM对其抗真菌和生防活性的贡献，我们将基因组分析与我们之前对转座子（Tn）突变体的研究相结合。通过抗真菌活性的降低，鉴定出13个Tn突变体，它们属于antiSMASH工具预测的总共14个生物合成基因簇（BGCs）中的6个BGCs。Pf275的生防性能显著依赖于2,4-二乙酰基间苯三酚和绿脓菌素。编码芳基多烯和一种未鉴定的小线性脂肽的基因簇影响抗真菌和生防活性。据我们所知，我们的研究首次确定了小线性脂肽和芳基多烯等SMs对生防效果的贡献。
