Shokri Meysam, Hazrati Tappeh Khosro, Meshkini Elyar, Aminpour Arash
Department of Parasitology and Mycology, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
Iran J Parasitol. 2020 Jul-Sep;15(3):349-356. doi: 10.18502/ijpa.v15i3.4199.
In this study, the effect of total lysate antigen (TLA) of on spleen lymphocyte prolifration, secretion of IL5, INF-γ, and mice survival time was evaluated using agonist of toll-like receptor (TLR) 11, as an adjuvant.
This study was done in the Department of Parasitology and Mycology of Urmia University of Medical Sciences, Urmia, Iran in 2018. First, different groups of BALB/c mice were immunized with TLA alone and also TLA + TLR11 agonist. Subsequently, factors such as spleen lymphocyte prolifration, IL-5, and INF-γ secretion and mice survival time in different groups were compared.
Mice immunized with TLA + adjuvant showed higher immunization index than the two other groups and combination of TLR11 (as an adjuvant) and TLA significantly elevated the effect of TLA by increasing the production of INF-γ and IL-5 and by the shift of the immune system to Th1. In addition, the combination of TLA and TLR11 adjuvant increased the proliferation of lymphocytes and survival time in mice against
Profilin (as an adjuvant) in combination with TLA could be a potent vaccine candidate that evokes a powerful specific immune response and significantly improves the efficacy of TLA vaccine by increasing the induction of INF-γ production and by shifting the immune responses to Th1 profile through increasing the INF-γ/IL-5 ratio. It causes significant protection against after i.p. injection.
在本研究中，使用 toll 样受体（TLR）11 激动剂作为佐剂，评估了 的全裂解物抗原（TLA）对脾淋巴细胞增殖、IL-5、INF-γ 分泌以及小鼠存活时间的影响。
本研究于 2018 年在伊朗乌尔米亚医科大学寄生虫学和真菌学系进行。首先，用单独的 TLA 以及 TLA + TLR11 激动剂对不同组的 BALB/c 小鼠进行免疫。随后，比较不同组中脾淋巴细胞增殖、IL-5 和 INF-γ 分泌以及小鼠存活时间等因素。
用 TLA + 佐剂免疫的小鼠显示出比其他两组更高的免疫指数，并且 TLR11（作为佐剂）与 TLA 的组合通过增加 INF-γ 和 IL-5 的产生以及使免疫系统向 Th1 偏移，显著提高了 TLA 的效果。此外，TLA 和 TLR11 佐剂的组合增加了小鼠淋巴细胞的增殖和存活时间。
肌动蛋白结合蛋白（作为佐剂）与 TLA 联合使用可能是一种有效的疫苗候选物，它能引发强大的特异性免疫反应，并通过增加 INF-γ 的产生诱导以及通过提高 INF-γ/IL-5 比值使免疫反应向 Th1 型转变，显著提高 TLA 疫苗的效力。经腹腔注射后，它能对 提供显著的保护。
