Wen Chengfei, Jiang Mi, Huang Weixin, Liu Shumei
Department of Cardiology, The First Hospital of Jilin University, Changchun 130021, China.
Department of Cardiac Surgery, The First Hospital of Jilin University, Changchun 130021, China.
Evid Based Complement Alternat Med. 2020 Oct 7;2020:9534137. doi: 10.1155/2020/9534137. eCollection 2020.
Antarctic krill oil (AKO) has strong antioxidant activities and is effective for alleviating coronary heart disease (CHD). Kelch-like ECH-associated protein 1-NF-E2-related factor 2 (KEAP1-NRF2) axis is a crucial antioxidant signaling pathway. Thus, AKO may exert its antioxidant effects on CHD patients via KEAP1-NRF2 signaling.
AKO fatty acid (FA) profiles were analyzed by using gas chromatography (GC). One hundred CHD patients were divided into the intervention (IG, AKO) and control (CG, placebo) groups. Before and after 1, 2, and 3 months of intervention, we measured serum levels of reactive oxygen species (ROS), 8-hydroxy-2-deoxyguanosine (8-OHdG), nitric oxide (NO), malondialdehyde (MDA), superoxide dismutase (SOD), reduced glutathione (GSH), and glutathione peroxidase (GPx), and KEAP1 and NRF2 levels in peripheral blood leukocytes (PBLs). Serum FAs were measured by GC at baseline and after 3-month intervention.
AKO contains rich eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), which is more than 27% of total FA. The levels of EPA and DHA, KEAP1, and NRF2 in the IG group were higher than those in the CG group ( < 0.05). Serum levels of ROS, 8-OHdG, NO, and MDA in the IG group were lower than those in the CG group, whereas the levels of SOD, GSH, and GPx in the IG group were higher than those in the CG group ( < 0.05). Serum levels of saturated fatty acids (UFA) in the IG group were higher than those in the CG group, whereas reverse results were obtained for the levels of saturated fatty acids (SFA). Serum levels of EPA and DHA had a strong negative relationship with the level of ROS, whereas the ROS level had a strong negative relationship with the levels of KEAP1-NRF2.
AKO increases antioxidant capacities of CHD patients via the KEAP1-NRF2 signaling in the PBL.
南极磷虾油（AKO）具有较强的抗氧化活性，对缓解冠心病（CHD）有效。 Kelch样ECH相关蛋白1-NF-E2相关因子2（KEAP1-NRF2）轴是一条关键的抗氧化信号通路。因此，AKO可能通过KEAP1-NRF2信号通路对冠心病患者发挥抗氧化作用。
采用气相色谱法（GC）分析AKO脂肪酸（FA）谱。将100例冠心病患者分为干预组（IG，AKO）和对照组（CG，安慰剂）。在干预1、2和3个月前后，我们测量了血清活性氧（ROS）、8-羟基-2-脱氧鸟苷（8-OHdG）、一氧化氮（NO）、丙二醛（MDA）、超氧化物歧化酶（SOD）、还原型谷胱甘肽（GSH）和谷胱甘肽过氧化物酶（GPx）水平，以及外周血白细胞（PBL）中KEAP1和NRF2水平。在基线和3个月干预后通过GC测量血清脂肪酸。
AKO含有丰富的二十碳五烯酸（EPA）和二十二碳六烯酸（DHA），占总脂肪酸的27%以上。IG组的EPA、DHA、KEAP1和NRF2水平高于CG组（<0.05）。IG组血清ROS、8-OHdG、NO和MDA水平低于CG组，而IG组SOD、GSH和GPx水平高于CG组（<0.05）。IG组血清不饱和脂肪酸（UFA）水平高于CG组，而饱和脂肪酸（SFA）水平则相反。血清EPA和DHA水平与ROS水平呈强负相关，而ROS水平与KEAP1-NRF2水平呈强负相关。
AKO通过PBL中的KEAP1-NRF2信号通路提高冠心病患者的抗氧化能力。
