Qiu Lifeng, Yao Lingya, Fang Yanfei, Wang Lan, Xue Meng, Lin Zhenghua, Chen Shujie, Si Jianmin
Department of General Practice, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou, Zhejiang 310000, China.
Institute of Gastroenterology, Zhejiang University, Hangzhou, Zhejiang 310000, China.
Evid Based Complement Alternat Med. 2020 Oct 7;2020:9592608. doi: 10.1155/2020/9592608. eCollection 2020.
The continuing use of nonsteroidal anti-inflammatory drugs (NSAIDs) usually increases the side effects such as peptic ulcer and acute gastric lesions in the gastrointestinal tract. Cuttlebone (CB), isolated from Sepiella maindroni de Rochebrune, was reported to have antioxidant activities, but its role in the treatment of indomethacin-induced gastric lesions has not yet been confirmed. In this research, we investigate the protective effect of cuttlebone on indomethacin-related ulcers in rats and possible mechanisms. Here, gastric ulcers were induced by oral administration of indomethacin, and then the rats were treated with omeprazole (4 mg/kg) or different doses (750, 1500, and 3000 mg/kg of body weight) of cuttlebone. We evaluated lesion index, inflammation score, and a series of oxidant/antioxidant parameters. The data demonstrated that cuttlebone could protect against gastric ulcers induced by indomethacin in a dose-dependent manner (positive correlation). Also, these effects were associated with attenuating the expression of malonaldehyde (MDA) and increasing the levels of some protective ingredients like epidermal growth factor (EGF), prostaglandin E2 (PGE2), and superoxide dismutase (SOD). Thus, considering its ability to protect indomethacin-induced acute gastric mucosal lesions and the underlying mechanisms, CB might be a potential candidate for treating gastric damage caused by NSAIDs.
非甾体抗炎药（NSAIDs）的持续使用通常会增加胃肠道副作用，如消化性溃疡和急性胃损伤。据报道，从曼氏无针乌贼（Sepiella maindroni de Rochebrune）中分离出的海螵蛸（CB）具有抗氧化活性，但其在治疗吲哚美辛诱导的胃损伤中的作用尚未得到证实。在本研究中，我们研究了海螵蛸对大鼠吲哚美辛相关溃疡的保护作用及其可能的机制。在此，通过口服吲哚美辛诱导胃溃疡，然后用奥美拉唑（4 mg/kg）或不同剂量（750、1500和3000 mg/kg体重）的海螵蛸对大鼠进行治疗。我们评估了病变指数、炎症评分以及一系列氧化/抗氧化参数。数据表明，海螵蛸可以剂量依赖性方式（正相关）预防吲哚美辛诱导的胃溃疡。此外，这些作用与丙二醛（MDA）表达的减弱以及表皮生长因子（EGF）、前列腺素E2（PGE2）和超氧化物歧化酶（SOD）等一些保护成分水平的增加有关。因此，考虑到其保护吲哚美辛诱导的急性胃黏膜损伤的能力及其潜在机制，海螵蛸可能是治疗NSAIDs引起的胃损伤的潜在候选药物。
