Department of Pediatric Neurology, The First Hospital of Jilin University, Changchun, Jilin 130021, China.
Department of Cardiology, The China-Japan Union Hospital of Jilin University, Changchun, Jilin 130031, China.
Dis Markers. 2020 Oct 7;2020:8840482. doi: 10.1155/2020/8840482. eCollection 2020.
Rolandic epilepsy is one of the most common epileptic syndromes in childhood. We used TMT-based proteomics and bioinformatics analysis to identify the differentially expressed proteins in plasma of children with Rolandic epilepsy. Our aim was to provide a molecular basis for exploring possible mechanisms underlying the pathogenesis of epilepsy. Subjects were divided into two groups (five in each): patients with Rolandic epilepsy as cases and patients with migraine as controls. Total proteins were extracted and quantitatively labeled with TMT, then analyzed using liquid chromatography mass spectrometry. Bioinformatics analysis was used to identify the hub genes. A total of 752 proteins were identified, of which 670 contained quantitative proteins. 217 differentially expressed proteins were identified, 46 of which were only upregulated in more than two groups and 111 of which were only downregulated in more than two groups. Bioinformatics analysis revealed top 10 hub genes in the up- and downregulated groups, respectively. Our study demonstrates that some differentially expressed proteins are associated with epilepsy. Activation of acute-phase or innate immune response and complement and fibrinogen systems and repression of glycolysis, lipoprotein metabolism, and antioxidant activity may play a role in the development of epilepsy.
Rolandic 癫痫是儿童最常见的癫痫综合征之一。我们使用基于 TMT 的蛋白质组学和生物信息学分析来鉴定 Rolandic 癫痫儿童血浆中的差异表达蛋白。我们的目的是为探索癫痫发病机制的可能机制提供分子基础。受试者分为两组（每组五人）：病例为 Rolandic 癫痫患者，对照为偏头痛患者。提取总蛋白并用 TMT 进行定量标记，然后使用液相色谱-质谱联用进行分析。生物信息学分析用于鉴定枢纽基因。共鉴定出 752 种蛋白质，其中 670 种含有定量蛋白质。鉴定出 217 种差异表达蛋白，其中 46 种在两组以上上调，111 种在两组以上下调。生物信息学分析分别揭示了上调和下调组中的前 10 个枢纽基因。本研究表明，一些差异表达蛋白与癫痫有关。急性期或先天免疫反应和补体及纤维蛋白系统的激活以及糖酵解、脂蛋白代谢和抗氧化活性的抑制可能在癫痫的发生发展中起作用。
