新型产聚 -L- 赖氨酸乳酸菌的优化生产来自传统乳制品。

Optimization of the Production of -Poly-L-Lysine by Novel Producer Lactic Acid Bacteria Isolated from Traditional Dairy Products.

机构信息

Department of Food Science and Technology, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran.

Department of Plant Breeding and Biotechnology, Faculty of Agricultural Engineering, Shahrood University of Technology, Shahrood, Iran.

出版信息

Biomed Res Int. 2020 Oct 5;2020:2145656. doi: 10.1155/2020/2145656. eCollection 2020.

New strains of lactic acid bacteria (LAB) were isolated from different traditional dairy products. Six new strains named strain A01, subsp. strain D01, subsp. strain E01, strain G01, strain C01, and subsp. strain F01 were identified using 16S rDNA sequencing, morphological and biochemical traits. All strains have been registered in the National Center for Biotechnology Information (NCBI) with accession numbers MN611241.1, MN611300.1, MN611301.1, MN611303.1, MN611241.1, and MN611299.1, respectively. Having found -Poly-L-Lysine (-PL) in all strains isolated, strain A01 was identified as an active producer of -Poly-L-Lysine (-PL). The one-factor-at-a-time method and central composite design were applied to optimize -Poly-L-Lysine (-PL). A predicted 200 ppm of -PL was obtained in the medium containing the lowest level of glucose, 25 g/l, and yeast extract, 6 g/l.

从不同传统乳制品中分离出新的乳酸菌 (LAB) 菌株。使用 16S rDNA 测序、形态和生化特征鉴定了 6 株新菌株，分别命名为株 A01、亚种株 D01、亚种株 E01、株 G01、株 C01 和亚种株 F01。所有菌株均已在国家生物技术信息中心 (NCBI) 注册，登录号分别为 MN611241.1、MN611300.1、MN611301.1、MN611303.1、MN611241.1 和 MN611299.1。在分离的所有菌株中均发现 -聚赖氨酸 (-PL)，株 A01 被鉴定为 -聚赖氨酸 (-PL) 的活性产生菌。采用单因素法和中心复合设计优化了 -聚赖氨酸 (-PL)。在含葡萄糖最低水平 25 g/L 和酵母提取物 6 g/L 的培养基中，获得了预测的 200 ppm 的 -PL。