Biotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, 88400, Kota Kinabalu, Sabah, Malaysia.
Can J Microbiol. 2021 Jan;67(1):64-74. doi: 10.1139/cjm-2019-0461. Epub 2020 Oct 21.
The effects of global warming are increasingly evident, where global surface temperatures and atmospheric concentration of carbon dioxide have increased in past decades. Given the role of terrestrial bacteria in various ecological functions, it is important to understand how terrestrial bacteria would respond towards higher environmental temperatures. This study aims to determine soil bacterial diversity in the tropics and their response towards in situ warming using an open-top chamber (OTC). OTCs were set up in areas exposed to sunlight throughout the year in the tropical region in Malaysia. Soil samples were collected every 3 months to monitor changes in bacterial diversity using V3-V4 16S rDNA amplicon sequencing inside the OTCs (treatment plots) and outside the OTCs (control plots). After 12 months of simulated warming, an average increase of 0.81 to 1.15 °C was recorded in treatment plots. Significant changes in the relative abundance of bacterial phyla such as and were reported. Increases in the relative abundance of were also observed in treatment plots after 12 months. Substantial changes were observed at the genus level, where most bacterial genera decreased in relative abundance after 12 months. This study demonstrated that warming can alter soil bacteria in tropical soils from Kota Kinabalu.
全球变暖的影响日益明显，过去几十年全球地表温度和大气二氧化碳浓度都有所上升。鉴于陆地细菌在各种生态功能中的作用，了解陆地细菌对更高环境温度的反应非常重要。本研究旨在使用开顶式气室 (OTC) 确定热带地区的土壤细菌多样性及其对原位变暖的反应。OTC 全年在马来西亚热带地区阳光充足的地区设立。每隔 3 个月采集土壤样本，使用 V3-V4 16S rDNA 扩增子测序在 OTC 内（处理地块）和 OTC 外（对照地块）监测细菌多样性的变化。在模拟升温 12 个月后，处理地块的平均温度升高了 0.81 至 1.15°C。据报道，细菌门的相对丰度发生了显著变化，如 和 。处理地块在 12 个月后也观察到 的相对丰度增加。在属水平上观察到了大量变化，大多数细菌属在 12 个月后相对丰度下降。本研究表明，变暖可以改变哥打京那巴鲁热带土壤中的土壤细菌。
