Universidade Estadual do Maranhão - UEMA, São Luís, MA, Brasil.
Laboratório de Imunoparasitologia, Departamento de Patologia, Reprodução e Saúde Única, Faculdade de Ciências Agrárias e Veterinárias - FCAV, Universidade Estadual Paulista "Júlio de Mesquita Filho" - UNESP, Jaboticabal, SP, Brasil.
Rev Bras Parasitol Vet. 2020 Oct 19;29(4):e014420. doi: 10.1590/S1984-29612020088. eCollection 2020.
Bartonella is a genus of emerging zoonotic bacteria that are mainly associated with mammalian erythrocytes and endothelial cells. Bats are natural reservoirs for a variety of important pathogens that impact human and animal health. Recent reports have highlighted the role of bats and bat flies in the maintenance of Bartonella. Here, we showed that none of the 29 bat DNA blood samples obtained from five bat species in São Luís Island, state of Maranhão, northeastern Brazil, were positive for Bartonella in qPCR assays targeting nuoG. On the other hand, three out of 15 DNA samples (20%) from flies in the family Streblidae were positive for Bartonella. The BLASTn results showed that the gltA and rpoB sequences shared identities ranging from 97.2% to 100%, with Bartonella sequences amplified from bats or bat flies from Costa Rica and Brazil. These findings were supported by phylogenetic analyses based on Bayesian inferences. The present study showed that Bartonella genotypes are present in bat flies, thus shedding some light on the distribution of bat fly-related Bartonella genotypes in South America.
巴尔通体是一类新兴的人畜共患病细菌属，主要与哺乳动物的红细胞和内皮细胞有关。蝙蝠是多种重要病原体的天然宿主，这些病原体对人类和动物的健康都有影响。最近的报告强调了蝙蝠和蝠蝇在巴尔通体维持中的作用。在这里，我们表明，在针对 nuoG 的 qPCR 检测中，从巴西东北部马拉尼昂州圣路易斯岛的五种蝙蝠中获得的 29 个蝙蝠 DNA 血液样本均未检测到巴尔通体阳性。另一方面，在 Streblidae 科的 15 个蝇 DNA 样本中，有 3 个（20%）对巴尔通体呈阳性。BLASTn 结果表明，gltA 和 rpoB 序列的同一性在 97.2%至 100%之间，与从哥斯达黎加和巴西蝙蝠或蝠蝇扩增的巴尔通体序列一致。基于贝叶斯推断的系统发育分析支持了这些发现。本研究表明，巴尔通体基因型存在于蝠蝇中，从而揭示了南美蝠蝇相关巴尔通体基因型的分布情况。
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