Fu Han-Bin, Luo Lin
Dept. of Stomatology, Wuhan Wuchang Hospital, Wuhan 430063, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2020 Oct 1;38(5):509-512. doi: 10.7518/hxkq.2020.05.006.
This study aimed to investigate the expression of HOX transcript antisense intergenic RNA (HOTAIR) in salivary adenoid cystic carcinoma (SACC) and explore its influence on prognosis.
A total of 86 cases of patients with SACC who underwent surgical resection treatment from March 2007 to March 2014 were selected. In the same period, 45 cases of normal salivary gland tissues were obtained. The expression of HOTAIR was detected via real-time fluorescent quantitative polymerase chain reaction. The patients were followed up after the surgery, and the follow-up deadline was March 31, 2019. The deaths and survival times of patients were recorded. Based on the quartile value of the relative expression level of HOTAIR in SACC patients, the patients were divided into low expression group and high expression group. Kaplan-Meier method and Log-Rank test were used to compare the survival time of the two groups. Taking the age, sex, tumor location, pathological type, tumor diameter, TNM stage, nerve invasion and lymph node metastasis as independent variables, Cox proportional hazards regression model was used to analyze the multiple factors affecting survival time.
The relative expression of HOTAIR in SACC tissue was 2.48±0.22, which was higher than that in normal salivary gland tissue at 1.03±0.13, and the difference was statistically significant (t=39.812, P<0.001). No nerve invasion and lymph node metastasis were observed in these patients compared with those patients with TNM stages Ⅰ or Ⅱ, while the relative expression of HOTAIR in the tissues of patients with TNM stages Ⅲ or Ⅳ, nerve invasion, and lymph node metastasis increased (P<0.05). Kaplan-Meier survival analysis showed that the average survival time and cumulative survival rate in the low expression group were higher than those in the high expression group [(113.32±10.77) months vs. (59.75±6.50) months and 72.73% vs. 39.06%, respectively, P=0.004]. Cox proportional hazard regression analysis showed that nerve invasion, lymph node metastasis, and the high expression of HOTAIR were the inde-pendent risk factors for the prognosis of patients with SACC (HR=3.274, 2.971, and 2.911, respectively, P<0.05).
HOTAIR was highly expressed in patients with SACC tissues and associated with poor prognosis. It is a risk factor for prog-nosis, and it is expected to be a potential marker for the prognostic assessment of patients with SACC.
本研究旨在探讨HOX转录反义基因间RNA（HOTAIR）在涎腺腺样囊性癌（SACC）中的表达情况，并探讨其对预后的影响。
选取2007年3月至2014年3月期间接受手术切除治疗的86例SACC患者。同期获取45例正常涎腺组织。通过实时荧光定量聚合酶链反应检测HOTAIR的表达。术后对患者进行随访，随访截止日期为2019年3月31日。记录患者的死亡情况和生存时间。根据SACC患者中HOTAIR相对表达水平的四分位数，将患者分为低表达组和高表达组。采用Kaplan-Meier法和Log-Rank检验比较两组的生存时间。以年龄、性别、肿瘤部位、病理类型、肿瘤直径、TNM分期、神经侵犯和淋巴结转移作为自变量，采用Cox比例风险回归模型分析影响生存时间的多因素。
SACC组织中HOTAIR的相对表达量为2.48±0.22，高于正常涎腺组织的1.03±0.13，差异有统计学意义（t=39.812，P<0.001）。与TNM Ⅰ期或Ⅱ期患者相比，这些患者未观察到神经侵犯和淋巴结转移，而TNM Ⅲ期或Ⅳ期、有神经侵犯和有淋巴结转移患者组织中HOTAIR的相对表达量增加（P<0.05）。Kaplan-Meier生存分析显示，低表达组的平均生存时间和累积生存率高于高表达组[分别为(113.32±10.77)个月对(59.75±6.50)个月和72.73%对39.06%，P=0.004]。Cox比例风险回归分析显示，神经侵犯、淋巴结转移和HOTAIR高表达是SACC患者预后的独立危险因素（HR分别为3.274、2.971和2.911，P<0.05）。
HOTAIR在SACC患者组织中高表达，且与预后不良相关。它是预后的一个危险因素，有望成为SACC患者预后评估的潜在标志物。
