Zhu Su-Zhen, Zhang Li, Zhang Jing, Guo Yin-Long
State Key Laboratory of Organometallic Chemistry and National Center for Organic Mass Spectrometry in Shanghai, Center for Excellence in Molecular Synthesis, Shanghai Institute of Organic Chemistry, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 345 Lingling Road, Shanghai 200032, China.
Anal Chem. 2020 Nov 3;92(21):14633-14639. doi: 10.1021/acs.analchem.0c03127. Epub 2020 Oct 21.
Fingerprinting mass spectrometric analysis at atmospheric conditions has been realized using an arc plasma-based dissociation (APD) device. Because of its high energy, high temperature, and unique chemical reactivity, the thermal plasma can induce dissociation of neutral molecules or ions produced by atmospheric ion sources. Both even/odd electron (fragment) ions would be generated to provide fingerprinting structural information and molecular weight of the compounds simultaneously. Meanwhile, elimination and aromatization were observed as special dissociation patterns in this device, which can be applied in the differentiation of isomers. The good compatibility with atmospheric ion sources is demonstrated by coupling the device with nanoelectrospray ionization (nano-ESI) and zero volt paper spray ionization (PSI), respectively. With erythromycin as the tuning standard, informative dissociation spectra of various compounds can be reproducible, making it possible to establish an arc plasma-based dissociation spectra database. This device allows fingerprinting mass spectrometric analysis, with no need for harsh vacuum conditions and is promising for making a breakthrough in making up the deficiency of atmospheric ionization techniques.
利用基于电弧等离子体解离（APD）的装置实现了常压条件下的指纹质谱分析。由于热等离子体具有高能量、高温和独特的化学反应活性，它能够诱导常压离子源产生的中性分子或离子发生解离。偶数/奇数电子（碎片）离子都会产生，从而同时提供化合物的指纹结构信息和分子量。同时，在该装置中观察到消除和芳构化作为特殊的解离模式，可应用于异构体的区分。分别将该装置与纳米电喷雾电离（nano-ESI）和零伏纸喷雾电离（PSI）联用，证明了其与常压离子源的良好兼容性。以红霉素作为调谐标准，各种化合物的信息丰富的解离光谱具有可重复性，从而有可能建立基于电弧等离子体的解离光谱数据库。该装置允许进行指纹质谱分析，无需苛刻的真空条件，有望在弥补常压电离技术的不足方面取得突破。
