College of Information Science and Electronic Engineering, Zhejiang University, Hangzhou, 310027, P. R. China.
Department of Chemistry, Zhejiang University, Hangzhou, 310027, P. R. China.
Nat Commun. 2024 Jul 18;15(1):6075. doi: 10.1038/s41467-024-50322-z.
Although numerous ambient ionization mass spectroscopy technologies have been developed over the past 20 years to address diverse analytical circumstances, a single-ion source technique that can handle all analyte types is still lacking. Here, a wide-energy programmable microwave plasma-ionization mass spectrometry (WPMPI-MS) system is presented, through which MS analysis can achieve high coverage of substances with various characteristics by digitally regulating the microwave energy. In addition, ionization energy can be rapidly scanned using programmable waveforms, enabling the simultaneous detection of biomolecules, heavy metals, non-polar molecules, etc., in seconds. WPMPI-MS performs well in analyzing real samples, rapidly analyzing nine toxicological standards in one drop of serum, and demonstrating good quantification and liquid chromatography coupling capability. The WPMPI-MS has also been used to detect soil extracts, solid pharmaceuticals, and landfill leachate, further demonstrating its robust analytical capabilities for real samples. The prospective uses of the technology in biological and chemical analysis are extensive, and it is anticipated to emerge as a viable alternative to commercially available ion sources.
尽管在过去的 20 年中已经开发出了许多环境电离质谱技术来应对不同的分析情况，但仍然缺乏一种可以处理所有分析物类型的单一离子源技术。在这里，我们提出了一种宽能程控微波等离子体电离质谱（WPMPI-MS）系统，通过该系统可以通过数字调节微波能量来实现对具有各种特性的物质的高覆盖率 MS 分析。此外，还可以使用可编程波形快速扫描电离能，从而实现生物分子、重金属、非极性分子等的秒级同时检测。WPMPI-MS 在分析实际样品时表现出色，可在一滴血清中快速分析九种毒理学标准品，并具有良好的定量和液相色谱耦合能力。WPMPI-MS 还用于检测土壤提取物、固体药物和垃圾渗滤液，进一步证明了其对实际样品的强大分析能力。该技术在生物和化学分析中的应用前景广泛，有望成为商业可用离子源的可行替代品。
