Logothetopoulos J, Valiquette N, MacGregor D, Hsia T
Banting and Best Department of Medical Research, University of Toronto, Canada.
Diabetes. 1987 Oct;36(10):1116-23. doi: 10.2337/diab.36.10.1116.
Intravenous transfusion of concanavalin A-activated splenic cells from acutely diabetic BB or diabetic BB/hooded hybrid donor rats into 6- to 36-h-old neonate recipients of diabetes-prone and -resistant rat lines induced insulitis and in some severe diabetes. These effects were observed 10-20 days after the injection of the blasts. Focal lesions of insulitis were absent in neonates killed 1 and 3 days after the blast injection but were observed in neonates killed on the 5th and 8th day. As determined by autoradiography after the injection of [3H]thymidine-labeled blasts, numerous blast cells migrated and settled in various immature lymph nodes and in the spleen within 24 h after injection. Focal mononuclear infiltrations in the islets containing labeled and unlabeled cells were again observed on the 5th and 8th day but not on the 1st and 3rd day after injection. These experiments indicate that target-specific blasts undergo a short phase of proliferation and maturation in lymphoid organs of the recipients, before initiating the autoimmune process in the pancreatic islets. They further suggest that specific immune cells rather than humoral anti-islet antibodies are more likely to play the major role in this autoimmune animal model of diabetes.
将急性糖尿病BB大鼠或糖尿病BB/带帽杂种供体大鼠经伴刀豆球蛋白A激活的脾细胞静脉输注到6至36小时龄的易患糖尿病和抗糖尿病大鼠品系的新生受体中，可诱发胰岛炎，并在某些情况下导致严重糖尿病。在注射胚细胞后10至20天观察到这些效应。在注射胚细胞后1天和3天处死的新生大鼠中未发现胰岛炎的局灶性病变，但在第5天和第8天处死的新生大鼠中观察到了。通过注射[3H]胸腺嘧啶标记的胚细胞后的放射自显影测定，注射后24小时内，大量胚细胞迁移并定居在各种未成熟淋巴结和脾脏中。在注射后第5天和第8天再次观察到胰岛中有含标记和未标记细胞的局灶性单核浸润，但在注射后第1天和第3天未观察到。这些实验表明，靶特异性胚细胞在受体的淋巴器官中经历短暂的增殖和成熟阶段，然后才在胰岛中启动自身免疫过程。它们进一步表明，在这种糖尿病自身免疫动物模型中，特异性免疫细胞而非体液抗胰岛抗体更有可能起主要作用。
