Löhr M, Markholst H, Dyrberg T, Klöppel G, Oberholzer M, Lernmark A
Institute of Pathology, University of Hamburg, F.R.G.
Pancreas. 1989;4(1):95-100. doi: 10.1097/00006676-198902000-00015.
Immunocytochemistry combined with morphometry was used to test the hypothesis that insulitis and diabetes are preceded by quantitative changes in the pancreas of diabetes-prone BB (DPBB) rats. Diabetes-resistant BB (DRBB) rats of the w-subline served as controls. In the first part of the study rats aged 15, 30, 45, and 60 days were studied. At 60 days preceding both insulitis and onset of diabetes, the DPBB rats demonstrated lower volumes of the entire pancreas, the parenchyma, the endocrine pancreas, and beta, A, and D cells. Body weights of the DPBB rats were lower than the DRBB rats from 15 days of age on. In the second part of the study, DP and DRBB rats were coreared by a foster mother to obtain weight-matched animals. Morphometric analysis at 70 days of age revealed a reduction in both the beta cell volume density and volume whereas no differences were seen in other pancreatic parameters. These results indicate that the appearance of insulitis and the later onset of insulin-dependent diabetes are preceded by a reduction in beta cell volume.
采用免疫细胞化学结合形态计量学方法，验证易患糖尿病的BB（DPBB）大鼠在发生胰岛炎和糖尿病之前胰腺会出现定量变化这一假说。w亚系的抗糖尿病BB（DRBB）大鼠作为对照。在研究的第一部分，对15、30、45和60日龄的大鼠进行研究。在胰岛炎和糖尿病发作前60天，DPBB大鼠的整个胰腺、实质、内分泌胰腺以及β、A和D细胞的体积均较小。从15日龄起，DPBB大鼠的体重就低于DRBB大鼠。在研究的第二部分，由代孕母鼠饲养DPBB和DRBB大鼠以获得体重匹配的动物。70日龄时的形态计量学分析显示，β细胞体积密度和体积均降低，而其他胰腺参数未见差异。这些结果表明，在胰岛炎出现和随后发生胰岛素依赖型糖尿病之前，β细胞体积会减小。
