Delmée M, Debongnie J C, Warzée P, Mainguet P
Service de Gastroentérologie, Clinique Universitaire Saint-Luc, Université Catholique de Louvain, Bruxelles.
Gastroenterol Clin Biol. 1987 Aug-Sep;11(8-9):550-3.
The presence of Campylobacter pylori in duodenal ulcer was investigated in 26 patients. The method used was a sterile endoscopic sampling technique which forcasted and checked for contamination of the samples. A sterile catheter was introduced through the endoscope and then an infusion of saline was instituted. Once the catheter was in the intestinal lumen, the infused solution was aspirated for control of sterility. Semi-quantitative cultures of gastric and duodenal juices and of biopsies of the duodenal ulcer, the bulbar mucosa close to the ulcer and prepyloric antral mucosa were compared. Only two contaminations were detected with this method. C. pylori was isolated in one gastric and one duodenal sample only. It was isolated in 14 of 26 duodenal ulcers, 12 of 26 bulbar mucosa and 20 of 26 mucosal specimens. Our study established that the presence of C. pylori was very uncommon in gastric and duodenal juices even when the bacterium was present in the mucosal membrane in large quantities.
对26例十二指肠溃疡患者的幽门螺杆菌感染情况进行了调查。采用的方法是无菌内镜采样技术，该技术可预测并检查样本是否受到污染。通过内镜插入一根无菌导管，然后注入生理盐水。一旦导管进入肠腔，就抽吸注入的溶液以检查无菌状态。对胃液、十二指肠液以及十二指肠溃疡活检组织、溃疡附近的球部黏膜和幽门窦黏膜进行了半定量培养，并进行了比较。用这种方法仅检测到两例污染。仅在一份胃样本和一份十二指肠样本中分离出幽门螺杆菌。在26例十二指肠溃疡中有14例、26例球部黏膜中有12例、26例黏膜标本中有20例分离出幽门螺杆菌。我们的研究表明，即使黏膜中大量存在幽门螺杆菌，其在胃液和十二指肠液中的存在也非常少见。
